| Title: |
Glucagon secretory response to hypoglycaemia, adrenaline and carbachol in streptozotocindiabetic rats |
| Authors: |
AHRÉN, B; STERN, JS; GINGERICH, RL; CURRY, DL; HAVEL, PJ |
| Source: |
Acta Physiologica, vol 155, iss 2 |
| Publisher Information: |
eScholarship, University of California |
| Publication Year: |
1995 |
| Collection: |
University of California: eScholarship |
| Subject Terms: |
32 Biomedical and Clinical Sciences (for-2020); 3202 Clinical Sciences (for-2020); Autoimmune Disease (rcdc); Diabetes (rcdc); 2.1 Biological and endogenous factors (hrcs-rac); Metabolic and endocrine (hrcs-hc); Animals (mesh); Blood Glucose (mesh); Body Weight (mesh); Carbachol (mesh); Diabetes Mellitus; Experimental (mesh); Drinking (mesh); Eating (mesh); Epinephrine (mesh); Glucagon (mesh); Hypoglycemia (mesh); Male (mesh); Rats (mesh); Rats; Sprague-Dawley (mesh); Receptors; Cholinergic (mesh); ADRENALINE; CHOLINERGIC; DIABETES; GLUCAGON SECRETION; HYPOGLYCEMIA |
| Subject Geographic: |
215 - 221 |
| Description: |
Glucagon response to insulin-induced hypoglycaemia is impared in diabetes, but the mechanism is not established. Pancreatic A cell hyporesponsiveness to adrenergic or cholinergic stimulation could contribute to the impairment. We therefore compared the plasma glucagon responses to intravenous infusion of adrenaline (1200 ng kg(-1) min(-1) for 20 min) or to intravenous injection of the cholinergic agonist carbachol (50 micrograms kg(-1)) in chloral hydrate-anaesthetized rats made diabetic with the use of streptozotocin (80 mg kg(-1) subcutaneously) 6 weeks before and in anaesthetized control rats. Insulin was infused intravenously to reduce plasma glucose levels to below 1.8 mmol L(-1). As expected, the plasma glucagon response was reduced by approximately 45% in streptozotocin-diabetic rats compared with controls (P = 0.045). During adrenaline infusion, plasma glucagon levels increased by 277 +/- 92 pg mL(-1) in controls (P = 0.009) and by 570 +/- 137 pg mL(-1) in the diabetic rats (P = 0.002). Thus, the plasma glucagon response to adrenaline was approximately doubled in the diabetic rats (P = 0.045). Following carbachol injection, plasma glucagon levels were raised by 1211 +/- 208 pg mL(-1) (P < 0.001) in controls but only by 555 +/- 242 pg mL(-1) in the diabetic rats (P = 0.049). Thus, the plasma glucagon response to carbachol was impared by approximately 58% in the diabetic rats (P = 0.028). We conclude that carbachol-stimulated glucagon secretion is impared concomitantly with the impared glucagon response to hypoglycaemia in streptozotocin-diabetic rats, whereas adrenaline-induced glucagon secretion is exaggerated. We suggest that a reduced pancreatic A cell responsiveness to cholinergic stimulation could contribute to the impairment of the glucagon response to insulin-induced hypoglycaemia in diabetes. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
unknown |
| Relation: |
qt3r59c5qm; https://escholarship.org/uc/item/3r59c5qm; https://escholarship.org/content/qt3r59c5qm/qt3r59c5qm.pdf |
| DOI: |
10.1111/j.1748-1716.1995.tb09966.x |
| Availability: |
https://escholarship.org/uc/item/3r59c5qm; https://escholarship.org/content/qt3r59c5qm/qt3r59c5qm.pdf; https://doi.org/10.1111/j.1748-1716.1995.tb09966.x |
| Rights: |
public |
| Accession Number: |
edsbas.A4D19552 |
| Database: |
BASE |