| Title: |
DNA barcoding and surveillance sampling strategies for Culicoides biting midges (Diptera: Ceratopogonidae) in southern India |
| Authors: |
Harrup, Lara E.; Laban, Swathi; Purse, Bethan V.; Reddy, Yarabolu Krishnamohan; Reddy, Yella Narasimha; Byregowda, Sonnahallipura Munivenkatappa; Kumar, Naveen; Purushotham, Kondappa Muniramaiah; Kowalli, Shrikant; Prasad, Minakshi; Prasad, Gaya; Bettis, Alison A.; De Keyser, Rien; Logan, James; Garros, Claire; Gopurenko, David; Bellis, Glenn; Labuschagne, Karien; Mathieu, Bruno; Carpenter, Simon |
| Publisher Information: |
BioMed Central |
| Publication Year: |
2016 |
| Collection: |
University of Pretoria: UPSpace |
| Subject Terms: |
Culicoides; Arbovirus; DNA barcodes; Surveillance; Bluetongue virus (BTV); cytochrome c oxidase I (COI); Light-emitting diodes (LEDs); Barcode of Life Data System (BOLD) |
| Description: |
Additional file 1: Table S1. GenBank sequences used in genetic analyses of Culicoides from southern India. Table S2. Barcode Index Numbers (BINs) assigned within the Barcode of Life Database (BOLD) for specimens collected within this study. ; Additional file 2: Table S3. Uncorrected percentage sequence distances, mean with range shown in parentheses. Intraspecific distances are shown in bold along the diagonal, interspecific distances are shown in the lower triangle (NA indicates comparison not possible due to singleton specimen present; number of specimens per species (n) shown in brackets with the number of specimens originating from this study; followed by the number originating from GenBank in parentheses). ; BACKGROUND : Culicoides spp. biting midges transmit bluetongue virus (BTV), the aetiological agent of bluetongue (BT), an economically important disease of ruminants. In southern India, hyperendemic outbreaks of BT exert high cost to subsistence farmers in the region, impacting on sheep production. Effective Culicoides spp. monitoring methods coupled with accurate species identification can accelerate responses for minimising BT outbreaks. Here, we assessed the utility of sampling methods and DNA barcoding for detection and identification of Culicoides spp. in southern India, in order to provide an informed basis for future monitoring of their populations in the region. METHODS : Culicoides spp. collected from Tamil Nadu and Karnataka were used to construct a framework for future morphological identification in surveillance, based on sequence comparison of the DNA barcode region of the mitochondrial cytochrome c oxidase I (COI) gene and achieving quality standards defined by the Barcode of Life initiative. Pairwise catches of Culicoides spp. were compared in diversity and abundance between green (570 nm) and ultraviolet (UV) (390 nm) light emitting diode (LED) suction traps at a single site in Chennai, Tamil Nadu over 20 nights of sampling in November 2013. RESULTS : DNA barcode sequences of Culicoides ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf; application/vnd.openxmlformats-officedocument.wordprocessingml.document; application/vnd.openxmlformats-officedocument.spreadsheetml.sheet |
| Language: |
English |
| Relation: |
http://hdl.handle.net/2263/56733 |
| DOI: |
10.1186/s13071-016-1722-z |
| Availability: |
http://hdl.handle.net/2263/56733; https://doi.org/10.1186/s13071-016-1722-z |
| Rights: |
© 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License. |
| Accession Number: |
edsbas.A5728716 |
| Database: |
BASE |