| Title: |
Image_1_A rapid point-of-care population-scale dipstick assay to identify and differentiate SARS-CoV-2 variants in COVID-19-positive patients.TIFF |
| Authors: |
Deepjyoti Paul; Jyoti Verma; Shakti Kumar; Daizee Talukdar; Pradipta Jana; Lekshmi Narendrakumar; Roshan Kumar; Subhash Tanwar; Mudita Gosain; Sonali Porey Karmakar; Madhu Pareek; Shailendra Mani; Susmita Chaudhuri; Pallavi Kshetrapal; Nitya Wadhwa; Shinjini Bhatnagar; Pramod Kumar Garg; Bhabatosh Das |
| Publication Year: |
2024 |
| Collection: |
Frontiers: Figshare |
| Subject Terms: |
Microbiology; Microbial Genetics; Microbial Ecology; Mycology; SARS-CoV-2; COVID-19; next-generation sequencing; multiplex-PCR; dipstick assay; rapid detection |
| Description: |
Delta and Omicron variants of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) are remarkably contagious, and have been recognized as variants of concern (VOC). The acquisition of spontaneous substitutions or insertion–deletion mutations (indels) in the spike protein-encoding gene substantially increases the binding affinity of the receptor binding domain (RBD)-hACE2 complex and upsurges the transmission of both variants. In this study, we analyzed thousands of genome sequences from 30 distinct SARS-CoV-2 variants, focusing on the unique nucleic acid signatures in the spike gene specific to the Delta and Omicron variants. Using these variant-specific sequences, we synthesized a range of oligonucleotides and optimized a multiplex PCR (mPCR) assay capable of accurately identifying and differentiating between the Delta and Omicron variants. Building on this mPCR assay, we developed a dipstick format by incorporating a tag linker sequence at the 5′ end of the forward primer and adding biotin to the 3′ end of the oligonucleotides, enhancing the assay’s usability and accessibility. Streptavidin-coated latex beads and the dipstick imprinted with a probe for the tag linker sequence in the test strips were used for the detection assay. Our dipstick-based assay, developed as a rapid point-of-care test for identifying and differentiating SARS-CoV-2 variants has the potential to be used in low-resource settings and scaled up to the population level. |
| Document Type: |
still image |
| Language: |
unknown |
| DOI: |
10.3389/fmicb.2024.1459644.s001 |
| Availability: |
https://doi.org/10.3389/fmicb.2024.1459644.s001; https://figshare.com/articles/figure/Image_1_A_rapid_point-of-care_population-scale_dipstick_assay_to_identify_and_differentiate_SARS-CoV-2_variants_in_COVID-19-positive_patients_TIFF/27265857 |
| Rights: |
CC BY 4.0 |
| Accession Number: |
edsbas.A5C4B03E |
| Database: |
BASE |