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Gliotoxin Is a Dual Inhibitor of Farnesyltransferase and Geranylgeranyltransferase I with Antitumor Activity Against Breast Cancer In Vivo

Title: Gliotoxin Is a Dual Inhibitor of Farnesyltransferase and Geranylgeranyltransferase I with Antitumor Activity Against Breast Cancer In Vivo
Authors: Vigushin, DM; Mirsaidi, N; Brooke, G; Sun, C; Pace, P; Inman, L; Moody, CJ; Coombes, RC
Publisher Information: Springer Science and Business Media LLC
Publication Year: 2004
Collection: University of Essex Research Repository
Subject Terms: RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Description: Gliotoxin is a natural mycotoxin with immunosuppressive and antimicrobial activity. Inhibition of farnesyltransferase (IC 50 80 μM) and geranylgeranyltransferase I (IC 50 17 μM) stimulated interest in the potential antitumor activity of this epidithiodioxopiperazine. Gliotoxin inhibited proliferation of six breast cancer cell lines in culture with mean ± SD IC 50 289 ± 328 μM (range 38-985 μM); intracellular farnesylation of Lamin B and geranylgeranylation of Rap1A were inhibited in a dose-dependent manner. In randomized controlled studies using the N-methyl-N-nitrosourea rat mammary carcinoma model, gliotoxin had pronounced antitumor activity in vitro and little systemic toxicity when administered to 10 animals at 10 mg/kg by subcutaneous injection weekly for 4 wk compared with 10 controls. Single doses up to 25 mg/kg were well tolerated. The present studies confirm that gliotoxin is a dual inhibitor of farnesyltransferase and geranylgeranyltransferase I with pronounced antitumor activity and favorable toxicity profile against breast cancer in vitro and in vivo.
Document Type: article in journal/newspaper
Language: unknown
Relation: Vigushin, DM and Mirsaidi, N and Brooke, G and Sun, C and Pace, P and Inman, L and Moody, CJ and Coombes, RC (2004) Gliotoxin Is a Dual Inhibitor of Farnesyltransferase and Geranylgeranyltransferase I with Antitumor Activity Against Breast Cancer In Vivo. Medical Oncology, 21 (1). pp. 21-30. DOI https://doi.org/10.1385/mo:21:1:21
DOI: 10.1385/mo:21:1:21
Availability: https://repository.essex.ac.uk/8309/; https://doi.org/10.1385/mo:21:1:21
Accession Number: edsbas.A75F8480
Database: BASE