| Title: |
Mitochondrial DNA oxidation propagates autoimmunity by enabling plasmacytoid dendritic cells induce Tfh differentiation |
| Authors: |
Karin, Michael; Xian, Hongxu; Ohira, Masafumi; Watari, Kosuke; Brito, Jonathan; Onyuru, Janset; Zuniga, Elina; Hoffman, Hal |
| Publisher Information: |
Springer Science and Business Media LLC |
| Publication Year: |
2024 |
| Description: |
NLRP3 inflammasome activation depends on stress-induced production of oxidized mitochondrial DNA (Ox-mtDNA) fragments that enter the cytoplasm to bind NLRP3 and activate caspase-1. Along with pro-IL-1β processing, caspase-1 generates gasdermin D pores that result in circulatory mtDNA release. Elevated amounts of circulating cell-free (ccf)-mtDNA, which is likely to be oxidized, were documented in the elderly and patients with metabolic and autoimmune disorders and its intra-articular injection elicited arthritis in mice. Investigating whether ccf-mtDNA may promote autoimmunity, we found that induction of sustained Ox-mtDNA release triggered by a prototypical NLRP3 inflammasome activator elicited autoantibody production and glomerulonephritis in mice. Similar autoimmune responses, dependent on plasmacytoid dendritic cells (pDC) and T follicular helper cells (Tfh), were elicited by in-vitro generated Ox-mtDNA but not by non-oxidized mtDNA. Although both mtDNA forms were internalized by pDC and induced interferon-a, only Ox-mtDNA stimulated autocrine IL-1β signaling that induced expression of immunoregulatory and co-stimulatory molecules, including IL-21, that enabled mouse and human pDC convert naïve CD4 + T cells into functional Tfh, supportive of autoantibody production. Highlighting pDC-generated IL-1β as an orchestrator of autoantibody production, these findings suggest that Ox-mtDNA could be a key participant in immune-aging and unravel new therapeutic opportunities. |
| Document Type: |
other/unknown material |
| Language: |
unknown |
| DOI: |
10.21203/rs.3.rs-5194985/v1 |
| Availability: |
https://doi.org/10.21203/rs.3.rs-5194985/v1; https://www.researchsquare.com/article/rs-5194985/v1; https://www.researchsquare.com/article/rs-5194985/v1.html |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.A7DBA110 |
| Database: |
BASE |