| Title: |
MicroRNA 146a is associated with diabetic complications in type 1 diabetic patients from the EURODIAB PCS |
| Authors: |
Barutta F.; Corbetta B.; Bellini S.; Guarrera S.; Matullo G.; Scandella M.; Schalkwijk C.; Stehouwer C. D.; Chaturvedi N.; Soedamah-Muthu S. S.; Durazzo M.; Gruden G. |
| Contributors: |
Barutta F.; Corbetta B.; Bellini S.; Guarrera S.; Matullo G.; Scandella M.; Schalkwijk C.; Stehouwer C.D.; Chaturvedi N.; Soedamah-Muthu S.S.; Durazzo M.; Gruden G. |
| Publication Year: |
2021 |
| Collection: |
Università degli studi di Torino: AperTo (Archivio Istituzionale ad Accesso Aperto) |
| Subject Terms: |
Cardiovascular disease; MicroRNA; Retinopathy; Type 1 diabete; Case-Control Studie; Human; Tumor Necrosis Factor-alpha; Diabetes Mellitus; Type 1; Diabetic Retinopathy; MicroRNAs |
| Description: |
Background: MicroRNA-146a-5p (miR-146a-5p) is a key regulator of inflammatory processes. Expression of miR-146a-5p is altered in target organs of diabetic complications and deficiency of miR-146a-5p has been implicated in their pathogenesis. We investigated if serum miR-146a-5p levels were independently associated with micro/macrovascular complications of type 1 diabetes (DM1). Methods: A nested case–control study from the EURODIAB PCS of 447 DM1 patients was performed. Cases (n = 294) had one or more complications of diabetes, whereas controls (n = 153) did not have any complication. Total RNA was isolated from all subjects and miR-146a-5p levels measured by qPCR. Both the endogenous controls U6 snRNA and the spike (Cel-miR-39) were used to normalize the results. Logistic regression analysis was carried out to investigate the association of miR-146a-5p with diabetes complications. Results: MiR-146a-5p levels were significantly lower in cases [1.15 (0.32–3.34)] compared to controls [1.74 (0.44–6.74) P = 0.039]. Logistic regression analysis showed that levels of miR-146a-5p in the upper quartile were inversely associated with reduced odds ratio (OR) of all complications (OR 0.34 [95% CI 0.14–0.76]) and particularly with cardiovascular diseases (CVD) (OR 0.31 [95% CI 0.11–0.84]) and diabetic retinopathy (OR 0.40 [95% CI 0.16–0.99]), independently of age, sex, diabetes duration, A1c, hypertension, AER, eGFR, NT-proBNP, and TNF-α. Conclusions: In this large cohort of DM1 patients, we reported an inverse and independent association of miR-146a-5p with diabetes chronic complications and in particular with CVD and retinopathy, suggesting that miR-146a-5p may be a novel candidate biomarker of DM1 complications. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/34823560; info:eu-repo/semantics/altIdentifier/wos/WOS:000722620400002; volume:19; issue:1; firstpage:475; lastpage:484; numberofpages:10; journal:JOURNAL OF TRANSLATIONAL MEDICINE; http://hdl.handle.net/2318/1844237; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85120697920 |
| DOI: |
10.1186/s12967-021-03142-4 |
| Availability: |
http://hdl.handle.net/2318/1844237; https://doi.org/10.1186/s12967-021-03142-4 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.A8DADD66 |
| Database: |
BASE |