| Title: |
An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity |
| Authors: |
Calandrini,Camilla; Schutgens, Frans; Oka,Rurika; Margaritis, Thanasis; Candelli,Tito; Mathijsen,Luka; Ammerlaan, Carola; van Ineveld,Ravian L.; Derakhshan,Sepide; de Haan,Sanne; Dolman, Emmy; Lijnzaad,Philip; Custers,Lars; Begthel,Harry; Kerstens,Hindrik H.D.; Visser,Lindy L.; Rookmaaker, Maarten; Verhaar, Marianne; Tytgat,Godelieve A.M.; Kemmeren, Patrick; de Krijger, Ronald R.; Al-Saadi,Reem; Pritchard-Jones,Kathy; Kool,Marcel; Rios,Anne C.; van den Heuvel-Eibrink, Marry M.; Molenaar,Jan J.; van Boxtel, Ruben; Holstege, Frank C.P.; Clevers, Hans; Drost,Jarno; Nefro Vasculaire Geneeskunde; Groep Holstege; CMM Groep Klumperman; MS Nefrologie; Regenerative Medicine and Stem Cells; Circulatory Health; Pathologie Groep Blokx; Pathologie Pathologen staf; Child Health; CMM Groep Cuppen; Cancer; CMM Sectie Molecular Cancer Research; Hubrecht Institute with UMC |
| Publication Year: |
2020 |
| Subject Terms: |
General Chemistry; General Biochemistry,Genetics and Molecular Biology; General Physics and Astronomy; Journal Article |
| Description: |
Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently lacking. Here, we describe the first paediatric cancer organoid biobank. It contains tumour and matching normal kidney organoids from over 50 children with different subtypes of kidney cancer, including Wilms tumours, malignant rhabdoid tumours, renal cell carcinomas, and congenital mesoblastic nephromas. Paediatric kidney tumour organoids retain key properties of native tumours, useful for revealing patient-specific drug sensitivities. Using single cell RNA-sequencing and high resolution 3D imaging, we further demonstrate that organoid cultures derived from Wilms tumours consist of multiple different cell types, including epithelial, stromal and blastemal-like cells. Our organoid biobank captures the heterogeneity of paediatric kidney tumours, providing a representative collection of well-characterised models for basic cancer research, drug-screening and personalised medicine. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text/plain |
| Language: |
English |
| ISSN: |
2041-1723 |
| Relation: |
https://dspace.library.uu.nl/handle/1874/439527 |
| Availability: |
https://dspace.library.uu.nl/handle/1874/439527 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.A9786CF2 |
| Database: |
BASE |