| Title: |
Loop-Mediated Isothermal Amplification (LAMP): An Innovative Approach for the Environmental Monitoring of SARS-CoV-2 |
| Authors: |
Simona Spiteri; Federica Marino; Luna Girolamini; Maria Rosaria Pascale; Carlo Derelitto; Laura Caligaris; Simone Paghera; Sandra Cristino |
| Contributors: |
Spiteri, Simona; Marino, Federica; Girolamini, Luna; Pascale, MARIA ROSARIA; Derelitto, Carlo; Caligaris, Laura; Paghera, Simone; Cristino, Sandra |
| Publication Year: |
2024 |
| Collection: |
IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
| Subject Terms: |
SARS-CoV-2; COVID-19; RT-qPCR; RT-LAMP; environmental surveillance; public health |
| Description: |
The rapid and accurate detection of SARS-CoV-2 in environmental settings is crucial for effective public health management during the COVID-19 pandemic. This study compares the performance of the Reverse Transcription quantitative polymerase chain reaction (RT-qPCR) and the Reverse Transcription loop-mediated isothermal amplification (RT-LAMP) for SARS-CoV-2 detection from 100 surface samples collected in healthcare environments. The reference method, RT-qPCR, identified a percentage of 25% of positive samples, while RT-LAMP detected a percentage of 27% of positive surfaces. Our findings reveal a sensitivity of 32% and specificity of 75% for RT-LAMP, with a positive predictive value of 30% and a negative predictive value of 77%. The overall accuracy and concordance with RT-qPCR was 64% for both methods. Despite its lower sensitivity compared to RT-qPCR, RT-LAMP had an advantage due to its rapid screening and environmental surveillance, which is particularly useful for confirming negative results. These results underscore the potential of RT-LAMP not only as a valuable method in the environmental monitoring of SARS-CoV-2 but also as a system to control the sanitation process in ordinary and emergency conditions, providing further optimization and validation for its reliability in routine surveillance and outbreak response efforts. |
| Document Type: |
article in journal/newspaper |
| File Description: |
ELETTRONICO |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/39599574; info:eu-repo/semantics/altIdentifier/wos/WOS:001365867500001; volume:13; issue:11; firstpage:1; lastpage:12; numberofpages:12; journal:PATHOGENS; https://hdl.handle.net/11585/1000561 |
| DOI: |
10.3390/pathogens13111022 |
| Availability: |
https://hdl.handle.net/11585/1000561; https://doi.org/10.3390/pathogens13111022; https://www.mdpi.com/2076-0817/13/11/1022 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.AAE76901 |
| Database: |
BASE |