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Identification of circulating microRNAs for the differential diagnosis of Parkinson's disease and Multiple System Atrophy

Title: Identification of circulating microRNAs for the differential diagnosis of Parkinson's disease and Multiple System Atrophy
Authors: Annamaria Vallelunga; Marco Ragusa; Stefania Di Mauro; Tommaso Iannitti; Manuela Pilleri; Roberta Biundo; Luca Weis; Cinzia Di Pietro; Angela De Iuliis; Alessandra Nicoletti; Mario Zappia; Michele Purrello; Angelo Antonini
Contributors: Vallelunga, Annamaria; Ragusa, Marco; Di Mauro, Stefania; Iannitti, Tommaso; Pilleri, Manuela; Biundo, Roberta; Weis, Luca; Di Pietro, Cinzia; De Iuliis, Angela; Nicoletti, Alessandra; Zappia, Mario; Purrello, Michele; Antonini, Angelo
Publisher Information: Frontiers Research Foundation
Publication Year: 2014
Collection: Padua Research Archive (IRIS - Università degli Studi di Padova)
Subject Terms: Atypical parkinsonian disorder; Circulating microRNA; Early diagnosi; MicroRNA; Multiple system atrophy; Parkinson's disease
Description: Background: Parkinson's disease (PD) is a progressive neurodegenerative disorder which may be misdiagnosed with atypical conditions such as Multiple System Atrophy (MSA), due to overlapping clinical features. MicroRNAs (miRNAs) are small non-coding RNAs with a key role in post-transcriptional gene regulation. We hypothesized that identification of a distinct set of circulating miRNAs (cmiRNAs) could distinguish patients affected by PD from MSA and healthy individuals. Results. Using TaqMan Low Density Array technology, we analyzed 754 miRNAs and found 9 cmiRNAs differentially expressed in PD and MSA patients compared to healthy controls. We also validated a set of 4 differentially expressed cmiRNAs in PD and MSA patients vs. controls. More specifically, miR-339-5p was downregulated, whereas miR-223*, miR-324-3p, and mir-24 were upregulated in both diseases. We found cmiRNAs specifically deregulated in PD (downregulation of miR-30c and miR-148b) and in MSA (upregulation of miR-148b). Finally, comparing MSA and PD, we identified 3 upregulated cmiRNAs in MSA serum (miR-24, miR-34b, miR-148b). Conclusions. Our results suggest that cmiRNA signatures discriminate PD from MSA patients and healthy controls and may be considered specific, non-invasive biomarkers for differential diagnosis. © Ragusa, Di Mauro, Iannitti, Pilleri, Biundo, Weis, Di Pietro, De Iuliis, Nicoletti, Zappia, Purrello and Antonini.
Document Type: article in journal/newspaper
Language: English
Relation: info:eu-repo/semantics/altIdentifier/wos/WOS:000336956000001; volume:8; journal:FRONTIERS IN CELLULAR NEUROSCIENCE; https://hdl.handle.net/11577/3457736
DOI: 10.3389/fncel.2014.00156
Availability: https://hdl.handle.net/11577/3457736; https://doi.org/10.3389/fncel.2014.00156
Rights: info:eu-repo/semantics/openAccess ; license:Creative commons ; license uri:http://creativecommons.org/licenses/by/4.0/
Accession Number: edsbas.AB4AC13
Database: BASE