| Title: |
High Biofilm-Forming Multidrug-Resistant Salmonella Infantis Strains from the Poultry Production Chain |
| Authors: |
Musa L.; Toppi V.; Stefanetti V.; Spata N.; Rapi M. C.; Grilli G.; Addis M. F.; Di Giacinto G.; Franciosini M. P.; Casagrande Proietti P. |
| Contributors: |
Musa, L.; Toppi, V.; Stefanetti, V.; Spata, N.; Rapi, M. C.; Grilli, G.; Addis, M. F.; Di Giacinto, G.; Franciosini, M. P.; Casagrande Proietti, P. |
| Publication Year: |
2024 |
| Collection: |
IRIS Università degli Studi di Perugia |
| Subject Terms: |
Salmonella environmental persistence; Salmonella virulence factors; biofilm formation; biosecurity; disinfection; multidrug-resistant (MDR); rdar morphotype |
| Description: |
The ability of Salmonella species to adhere to surfaces and form biofilms, leading to persistent environmental reservoirs, might represent a direct link between environmental contamination and food processing contamination. The purpose of this study was to investigate the biofilm-forming ability of 80 multidrug-resistant (MDR) and extended-spectrum beta-lactamase (ESBL) producing Salmonella enterica serovar Infantis strains isolated from the broiler food chain production through whole genome sequencing (WGS), PCR, and morphotype association assays. Biofilm formation was quantified by testing the strains at two different temperatures, using 96-well polystyrene plates. The rough and dry colony (rdar) morphotype was assessed visually on Congo red agar (CRA) plates. Based on our results, all tested S. Infantis strains produced biofilm at 22 degrees C with an rdar morphotype, while at 37 degrees C, all the isolates tested negative, except one positive. Most isolates (58.75%) exhibited strong biofilm production, while 36.25% showed moderate production. Only 5 out of 80 (6.25%) were weak biofilm producers. WGS analysis showed the presence of the fim cluster (fimADF) and the csg cluster (csgBAC and csgDEFG), also described in S. Typhimurium, which are responsible for fimbriae production. PCR demonstrated the presence of csgD, csgB, and fimA in all 80 S. Infantis strains. To our knowledge, this is the first study comparing the effects of two different temperatures on the biofilm formation capacity of ESBL producing S. Infantis from the broiler production chain. This study highlights that the initial biofilm components, such as curli and cellulose, are specifically expressed at lower temperatures. It is important to emphasize that within the broiler farm, the environmental temperature ranges between 18-22 degrees C, which is the optimum temperature for in vitro biofilm formation by Salmonella spp. This temperature range facilitates the expression of biofilm-associated genes, contributing to the persistence of S. Infantis ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
ELETTRONICO |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/39061277; info:eu-repo/semantics/altIdentifier/wos/WOS:001277897100001; volume:13; issue:7; firstpage:1; lastpage:11; numberofpages:11; journal:ANTIBIOTICS; https://hdl.handle.net/11391/1582433 |
| DOI: |
10.3390/antibiotics13070595 |
| Availability: |
https://hdl.handle.net/11391/1582433; https://doi.org/10.3390/antibiotics13070595 |
| Accession Number: |
edsbas.AC23FACD |
| Database: |
BASE |