| Title: |
Novel Function of Bluetongue Virus NS3 Protein in Regulation of the MAPK/ERK Signaling Pathway |
| Authors: |
Kundlacz, Cindy; Pourcelot, Marie; Fablet, Aurore; Amaral Da Silva Moraes, Rayane; Léger, Thibaut; Morlet, Bastien; Viarouge, Cyril; Sailleau, Corinne; Turpaud, Mathilde; Gorlier, Axel; Breard, Emmanuel; Lecollinet, Sylvie; van Rijn, Piet A.; Zientara, Stephan; Vitour, Damien; Caignard, Grégory |
| Contributors: |
López, Susana; Laboratoire d'Excellence |
| Source: |
Journal of Virology ; volume 93, issue 16 ; ISSN 0022-538X 1098-5514 |
| Publisher Information: |
American Society for Microbiology |
| Publication Year: |
2019 |
| Description: |
Bluetongue virus (BTV) is responsible of the arthropod-borne disease bluetongue (BT) transmitted to ruminants by blood-feeding midges. In this report, we found that BTV, through its nonstructural protein NS3 (BTV-NS3), interacts with BRAF, a key component of the MAPK/ERK pathway. In response to growth factors, this pathway promotes cell survival and increases protein translation. We showed that BTV-NS3 enhances the MAPK/ERK pathway, and this activation is BRAF dependent. Treatment of MAPK/ERK pathway with the pharmacologic inhibitor U0126 impairs viral replication, suggesting that BTV manipulates this pathway for its own benefit. Our results illustrate, at the molecular level, how a single virulence factor has evolved to target a cellular function to increase its viral replication. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1128/jvi.00336-19 |
| DOI: |
10.1128/JVI.00336-19 |
| Availability: |
https://doi.org/10.1128/jvi.00336-19; https://journals.asm.org/doi/pdf/10.1128/JVI.00336-19 |
| Rights: |
https://journals.asm.org/non-commercial-tdm-license |
| Accession Number: |
edsbas.AC5C1CA8 |
| Database: |
BASE |