| Title: |
Proteomic Profiling of Tissue Exosomes Indicates Continuous Release of Malignant Exosomes in Urinary Bladder Cancer Patients, Even with Pathologically Undetectable Tumour |
| Authors: |
Eldh, Maria; Mints, Michael; Hiltbrunner, Stefanie; Ladjevardi, Sam; Alamdari, Farhood; Johansson, Markus; Jakubczyk, Tomasz; Veerman, Rosanne E.; Winqvist, Ola; Sherif, Amir; Gabrielsson, Susanne |
| Publisher Information: |
Uppsala universitet, Urologkirurgi; Karolinska Inst, Dept Med Solna, Div Immunol & Allergy, S-17164 Stockholm, Sweden.; Karolinska Inst, Dept Med Solna, Div Immunol & Allergy, S-17164 Stockholm, Sweden.;Umeå Univ, Dept Surg & Perioperat Sci Urol & Androl, S-90736 Umeå, Sweden.; Akademiska Univ Hosp, Dept Urol, S-75185 Uppsala, Sweden.; Vastmanland Hosp, Dept Urol, S-72189 Västerås, Sweden.; Umeå Univ, Sundsvall Hosp, Dept Urol, S-90736 Umeå, Sweden.; Lanssjukhuset Ryhov, Dept Urol, S-55305 Jönköping, Sweden.; Karolinska Univ Hosp, Dept Clin Immunol & Transfus Med, S-17176 Solna, Sweden.; Umeå Univ, Dept Surg & Perioperat Sci Urol & Androl, S-90736 Umeå, Sweden.; MDPI |
| Publication Year: |
2021 |
| Collection: |
Uppsala University: Publications (DiVA) |
| Subject Terms: |
extracellular vesicles; exosomes; urinary bladder neoplasms; neoadjuvant therapy; cystectomy; proteomics; Clinical Medicine; Klinisk medicin; Cancer and Oncology; Cancer och onkologi |
| Description: |
Simple Summary Urinary bladder cancer (UBC) has a high recurrence rate, and biomarkers for different treatment strategies are highly needed. This study investigated the release of nanovesicles called exosomes from urinary bladder tissue from tumour-proximal sites as well as tumour-distant sites in transurethrally resected (TUR-B) patients with or without preoperative neoadjuvant chemotherapy prior to ensuing radical cystectomy-all without remaining visible tumour after TUR-B. We show that cancer-promoting exosomes were detected from both sites, suggesting that the previous tumour has altered the whole bladder tissue into a cancer-supporting milieu. The exosomes may originate from remaining pathologically undetectable cancer cells or transformed epithelial cells, and the study supports the notion of exosomes as mediators of metastatic spread and as potential biomarkers. It also supports early and radical removal of the bladder in urinary bladder cancer patients. Invasive urothelial bladder cancer (UBC) has high recurrence rates even after radical cystectomy (RC). Exosomes are membrane-bound nanovesicles, which have been shown to contribute to carcinogenesis and metastasis. We previously showed that urinary exosomes display a malignant profile in UBC patients despite the absence of detectable tumour. Here, we investigated exosomes from sampling sites close to or distant from the former tumour, aiming to understand the effect of the tumour on the local milieu. Ten patients scheduled for cystectomy after transurethral bladder resection (TUR-B), without remaining detectable tumour, were included. Exosomes were isolated from tissue explants of both the previous tumour site and distant bladder tissue. Proteins were quantified by mass spectrometry in seven patients. Exosomes from the previous tumour site were enriched in inflammatory but not cancer-related pathways compared to distant tissue. However, the 69 most abundant proteins in tissue-derived exosomes regardless of site, 20 of which were also found in urinary ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
Cancers, 2072-6694, 2021, 13:13; PMID 34209558; ISI:000671043700001 |
| DOI: |
10.3390/cancers13133242 |
| Availability: |
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-452218; https://doi.org/10.3390/cancers13133242 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.ADC20373 |
| Database: |
BASE |