| Title: |
Human newborn monocytes demonstrate distinct bcg-induced primary and trained innate cytokine production and metabolic activation in vitro |
| Authors: |
Angelidou A.; Diray-Arce J.; Conti M. G.; Netea M. G.; Blok B. A.; Liu M.; Sanchez-Schmitz G.; Ozonoff A.; van Haren S. D.; Levy O. |
| Contributors: |
Angelidou, A.; Diray-Arce, J.; Conti, M. G.; Netea, M. G.; Blok, B. A.; Liu, M.; Sanchez-Schmitz, G.; Ozonoff, A.; van Haren, S. D.; Levy, O. |
| Publisher Information: |
Frontiers Research Foundation; Lausanne, CH |
| Publication Year: |
2021 |
| Collection: |
Sapienza Università di Roma: CINECA IRIS |
| Subject Terms: |
bacille calmette-guérin (bcg) vaccine; cord blood; cytokine; immunometabolism; lactate; newborn monocyte; trained immunity; activation; metabolic; bcg vaccine; human; immunity; innate; immunologic memory; infant; newborn; monocytes |
| Description: |
Background: Newborns exhibit distinct immune responses and are at high risk of infection. Neonatal immunization with BCG, the live attenuated vaccine against tuberculosis (TB), is associated with broad protection against a range of unrelated pathogens, possibly reflecting vaccine-induced training of innate immune cells (“innate memory”). However, little is known regarding the impact of age on BCG-induced innate responses. Objective: Establish an age-specific human monocyte in vitro training platform to characterize and compare BCG-induced primary and memory cytokine responses and immunometabolic shifts. Design/Methods: Human neonatal and adult CD33-selected monocytes were stimulated for 24h with RPMI (control) or BCG (Danish strain) in 10% autologous serum, washed and cultured for 5 additional days, prior to re-stimulation with the TLR4 agonist LPS for another 24h. Supernatants were collected at Day 1 (D1) to measure primary innate responses and at Day 7 (D7) to assess memory innate responses by ELISA and multiplex cytokine and chemokine assays. Lactate, a signature metabolite increased during trained immunity, was measured by colorimetric assay. Results: Cytokine production by human monocytes differed significantly by age at D1 (primary, BCG 1:750 and 1:100 vol/vol, p |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/34326836; info:eu-repo/semantics/altIdentifier/wos/WOS:000678660800001; volume:12; firstpage:1; lastpage:12; numberofpages:12; journal:FRONTIERS IN IMMUNOLOGY; https://hdl.handle.net/11573/1629166 |
| DOI: |
10.3389/fimmu.2021.674334 |
| Availability: |
https://hdl.handle.net/11573/1629166; https://doi.org/10.3389/fimmu.2021.674334 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.ADE65453 |
| Database: |
BASE |