| Title: |
High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications |
| Authors: |
Alter, Claudio L.; Detampel, Pascal; Schefer, Roman B.; Lotter, Claudia; Hauswirth, Patrick; Puligilla, Ramya D.; Weibel, Vera J.; Schenk, Susanne H.; Heusermann, Wolf; Schürz, Melanie; Meisner-Kober, Nicole; Palivan, Cornelia; Einfalt, Tomaž; Huwyler, Jörg |
| Publisher Information: |
Nature Research |
| Publication Year: |
2023 |
| Collection: |
University of Basel: edoc |
| Description: |
Extracellular vesicles (EVs) are highly interesting for the design of next-generation therapeutics. However, their preparation methods face challenges in standardization, yield, and reproducibility. Here, we describe a highly efficient and reproducible EV preparation method for monodisperse nano plasma membrane vesicles (nPMVs), which yields 10 to 100 times more particles per cell and hour than conventional EV preparation methods. nPMVs are produced by homogenizing giant plasma membrane vesicles following cell membrane blebbing and apoptotic body secretion induced by chemical stressors. nPMVs showed no significant differences compared to native EVs from the same cell line in cryo-TEM analysis, in vitro cellular interactions, and in vivo biodistribution studies in zebrafish larvae. Proteomics and lipidomics, on the other hand, suggested substantial differences consistent with the divergent origin of these two EV types and indicated that nPMVs primarily derive from apoptotic extracellular vesicles. nPMVs may provide an attractive source for developing EV-based pharmaceutical therapeutics. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISSN: |
2399-3642 |
| Relation: |
https://edoc.unibas.ch/94492/1/s42003-023-04859-2.pdf; Alter, Claudio L. and Detampel, Pascal and Schefer, Roman B. and Lotter, Claudia and Hauswirth, Patrick and Puligilla, Ramya D. and Weibel, Vera J. and Schenk, Susanne H. and Heusermann, Wolf and Schürz, Melanie and Meisner-Kober, Nicole and Palivan, Cornelia and Einfalt, Tomaž and Huwyler, Jörg. (2023) High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications. Communications biology, 6 (1). p. 478.; info:pmid/37137966; urn:ISSN:2399-3642 |
| Availability: |
https://edoc.unibas.ch/94492/ |
| Rights: |
cc_by ; info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.AEE672B5 |
| Database: |
BASE |