| Title: |
Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb |
| Authors: |
Wolff, C.; Tinevez, J.Y.; Pietzsch, T.; Stamataki, E.; Harich, B.; Guignard, L.; Preibisch, S.; Shorte, S.; Keller, P.J.; Tomancak, P.; Pavlopoulos, A. |
| Publisher Information: |
eLife Sciences Publications |
| Publication Year: |
2018 |
| Collection: |
Max-Delbrueck-Center for Molecular Medicine, Berlin: MDC Repository |
| Subject Terms: |
Function and Dysfunction of the Nervous System |
| Description: |
During development, coordinated cell behaviors orchestrate tissue and organ morphogenesis. Detailed descriptions of cell lineages and behaviors provide a powerful framework to elucidate the mechanisms of morphogenesis. To study the cellular basis of limb development, we imaged transgenic fluorescently-labeled embryos from the crustacean Parhyale hawaiensis with multi-view light-sheet microscopy at high spatiotemporal resolution over several days of embryogenesis. The cell lineage of outgrowing thoracic limbs was reconstructed at single-cell resolution with new software called Massive Multi-view Tracker (MaMuT). In silico clonal analyses suggested that the early limb primordium becomes subdivided into anterior-posterior and dorsal-ventral compartments whose boundaries intersect at the distal tip of the growing limb. Limb-bud formation is associated with spatial modulation of cell proliferation, while limb elongation is also driven by preferential orientation of cell divisions along the proximal-distal growth axis. Cellular reconstructions were predictive of the expression patterns of limb development genes including the BMP morphogen Decapentaplegic. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf; other |
| Language: |
English |
| Relation: |
https://edoc.mdc-berlin.de/id/eprint/17315/1/17315oa.pdf; https://edoc.mdc-berlin.de/id/eprint/17315/7/17315suppl.zip; Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb. Wolff, C., Tinevez, J.Y., Pietzsch, T., Stamataki, E., Harich, B., Guignard, L., Preibisch, S., Shorte, S., Keller, P.J., Tomancak, P. and Pavlopoulos, A. eLife 7 : e34410. 29 March 2018; PMID:29595475; https://doi.org/10.7554/eLife.34410 |
| DOI: |
10.7554/eLife.34410 |
| Availability: |
https://edoc.mdc-berlin.de/id/eprint/17315/; https://edoc.mdc-berlin.de/17315/; https://doi.org/10.7554/eLife.34410 |
| Rights: |
cc_by |
| Accession Number: |
edsbas.AFA1DAF8 |
| Database: |
BASE |