| Title: |
Molecular, functional and structural properties of the prolyl oligopeptidase of Trypanosoma cruzi (POP Tc80), which is required for parasite entry into mammalian cells |
| Authors: |
Bastos, I, M; Grellier, Philippe; Martins, N, F; Cadavid-Restrepo, G; de Souza-Ault, M, R; Augustyns, Koen; Teixeira, A, R; Schrével, J; Maigret, B; da Silveira, J, F; Santana, J, M |
| Contributors: |
Universidade de Brasilia = University of Brasilia Brasília (UnB); Molécules de Communication et Adaptation des Micro-organismes (MCAM); Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS); Universiteit Antwerpen = University of Antwerp; Laboratoire Lorrain de Recherche en Informatique et ses Applications (LORIA); Institut National de Recherche en Informatique et en Automatique (Inria)-CentraleSupélec-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS) |
| Source: |
ISSN: 0264-6021. |
| Publisher Information: |
CCSD; Portland Press |
| Publication Year: |
2005 |
| Collection: |
Université de Lorraine: HAL |
| Subject Terms: |
mammalian cell; prolyl oligopeptidase; structural modelling; Tc80 proteinase; Trypanosoma cruzi; trypomastigote; [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
| Description: |
International audience ; We have demonstrated that the 80 kDa POP Tc80 (prolyl oligo-peptidase of Trypanosoma cruzi) is involved in the process of cell invasion, since specific inhibitors block parasite entry into non-phagocytic mammalian host cells. In contrast with other POPs, POP Tc80 is capable of hydrolysing large substrates, such as fibro-nectin and native collagen. In this study, we present the cloning of the POPTc80 gene, whose deduced amino acid sequence shares considerable identity with other members of the POP family, mainly within its C-terminal portion that forms the catalytic domain. Southern-blot analysis indicated that POPTc80 is present as a single copy in the genome of the parasite. These results are consistent with mapping of POPTc80 to a single chromosome. The active recombinant protein (rPOP Tc80) displayed kinetic properties comparable with those of the native enzyme. Novel in-hibitors were assayed with rPOP Tc80, and the most efficient ones presented values of inhibition coefficient K i 1.52 nM. Infective parasites treated with these specific POP Tc80 inhibitors attached to the surface of mammalian host cells, but were incapable of infecting them. Structural modelling of POP Tc80, based on the crystallized porcine POP, suggested that POP Tc80 is composed of an α/β-hydrolase domain containing the catalytic triad Ser 548-Asp 631-His 667 and a seven-bladed β-propeller non-catalytic domain. Docking analysis suggests that triple-helical collagen access to the catalytic site of POP Tc80 occurs in the vicinity of the interface between the two domains. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1042/BJ20041049 |
| Availability: |
https://mnhn.hal.science/mnhn-02070125; https://doi.org/10.1042/BJ20041049 |
| Accession Number: |
edsbas.B046B666 |
| Database: |
BASE |