| Title: |
MicroRNA expression analysis in peripheral blood and soft-tissue of patients with periprosthetic hip infection: a prospective controlled pilot study |
| Authors: |
Alp Paksoy; Sebastian Meller; Florian Schwotzer; Philipp Moroder; Andrej Trampuz; Jan-Philipp Imiolczyk; Carsten Perka; Matthias Hackl; Fabian Plachel; Doruk Akgün |
| Source: |
Bone & Joint Open, Vol 5, Iss 6, Pp 479-488 (2024) |
| Publisher Information: |
The British Editorial Society of Bone & Joint Surgery |
| Publication Year: |
2024 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
biomarker; circulating microrna; periprosthetic hip joint infection; microrna signature; crp; microrna expression level; microrna profiling; Orthopedic surgery; RD701-811 |
| Description: |
Aims: Current diagnostic tools are not always able to effectively identify periprosthetic joint infections (PJIs). Recent studies suggest that circulating microRNAs (miRNAs) undergo changes under pathological conditions such as infection. The aim of this study was to analyze miRNA expression in hip arthroplasty PJI patients. Methods: This was a prospective pilot study, including 24 patients divided into three groups, with eight patients each undergoing revision of their hip arthroplasty due to aseptic reasons, and low- and high-grade PJI, respectively. The number of intraoperative samples and the incidence of positive cultures were recorded for each patient. Additionally, venous blood samples and periarticular tissue samples were collected from each patient to determine miRNA expressions between the groups. MiRNA screening was performed by small RNA-sequencing using the miRNA next generation sequencing (NGS) discovery (miND) pipeline. Results: Overall, several miRNAs in plasma and tissue were identified to be progressively deregulated according to ongoing PJI. When comparing the plasma samples, patients with a high-grade infection showed significantly higher expression levels for hsa-miR-21-3p, hsa-miR-1290, and hsa-miR-4488, and lower expression levels for hsa-miR-130a-3p and hsa-miR-451a compared to the aseptic group. Furthermore, the high-grade group showed a significantly higher regulated expression level of hsa-miR-1260a and lower expression levels for hsa-miR-26a-5p, hsa-miR-26b-5p, hsa-miR-148b-5p, hsa-miR-301a-3p, hsa-miR-451a, and hsa-miR-454-3p compared to the low-grade group. No significant differences were found between the low-grade and aseptic groups. When comparing the tissue samples, the high-grade group showed significantly higher expression levels for 23 different miRNAs and lower expression levels for hsa-miR-2110 and hsa-miR-3200-3p compared to the aseptic group. No significant differences were found in miRNA expression between the high- and low-grade groups, as well as between the low-grade ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://doaj.org/toc/2633-1462; https://doaj.org/article/af992c4bf85941178d6974a9c5e6afdc |
| DOI: |
10.1302/2633-1462.56.BJO-2023-0172.R2 |
| Availability: |
https://doi.org/10.1302/2633-1462.56.BJO-2023-0172.R2; https://doaj.org/article/af992c4bf85941178d6974a9c5e6afdc |
| Accession Number: |
edsbas.B1A35C2F |
| Database: |
BASE |