| Title: |
Inter-individual Variability for High Fat Diet Consumption in Inbred C57BL/6 Mice |
| Authors: |
De Francesco, Pablo Nicolás; Cornejo, María Paula; Barrile, Franco; García Romero, Guadalupe; Valdivia Torres, Lesly Spring; Andreoli, Maria Florencia; Perelló, Mario Carlos |
| Publication Year: |
2019 |
| Collection: |
Universidad Nacional de La Plata (UNLP): SeDiCI (Servicio de Difusión de la Creación Intelectual) |
| Subject Terms: |
Ciencias Exactas; Biología; Ciencias Médicas; food intake; inter-individuality; palatable foods; eating behaviors; obesity |
| Description: |
Since inbred C57BL/6 mice are known to show inter-individual phenotypic variability for some traits, we tested the hypothesis that inbred C57BL/6 mice display a different tendency to consume a high fat (HF) diet. For this purpose, we used a compilation of HF intake data from an experimental protocol in which satiated mice were exposed to a HF pellet every morning for 2-h over 4 consecutive days. We found that mice displayed a large degree of variability in HF intake. Since day 1 HF intake significantly correlated with HF intake in successive days, we applied a hierarchical clustering algorithm on HF intake measurements in days 2, 3, and 4 in order to classify mice into "low" or "high" HF intake groups. "Low" HF intake group showed a day 1 HF intake similar to that seen in mice exposed to regular chow, while "high" HF intake group showed a higher day 1 HF intake as compared to "low" HF intake group. Both groups of mice increased HF consumption over the successive days, but "high" HF intake group always displayed a higher HF consumption than the "low" HF intake group. As compared to "low" HF intake group, "high" HF intake group showed a higher number of dopamine neurons positive for c-Fos in the VTA after the last event of HF intake. Thus, inbred C57BL/6 mice show inter-individual variability for HF intake and such feature may be linked to a different response to the rewarding properties of the HF diet. ; Instituto Multidisciplinario de Biología Celular |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
http://sedici.unlp.edu.ar/handle/10915/124765 |
| Availability: |
http://sedici.unlp.edu.ar/handle/10915/124765 |
| Rights: |
http://creativecommons.org/licenses/by/4.0/ ; Creative Commons Attribution 4.0 International (CC BY 4.0) |
| Accession Number: |
edsbas.B1BCBA84 |
| Database: |
BASE |