| Title: |
Cytokine Expression by Human Macrophage-Like Cells Derived from the Monocytic Cell Line THP-1 Differs Between Treatment With Milk from Preterm- and Term-Delivering Mothers and Pasteurized Donor Milk |
| Authors: |
Veronique Demers-Mathieu; Robert K. Huston; David C. Dallas |
| Source: |
Molecules, Vol 25, Iss 2376, p 2376 (2020) |
| Publisher Information: |
MDPI AG |
| Publication Year: |
2020 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
tumor necrosis factor-α; interleukin-6; interleukin-10; preterm infant; immunomodulatory effect; immature immunity; Organic chemistry; QD241-441 |
| Description: |
Immunomodulatory proteins from human milk may enhance the protection and development of the infant’s gut. This study compared the immunomodulatory effects of treatment with milk from preterm-(PM) and term-delivering (TM) mothers and pasteurized donor milk (DM) on cytokine gene expression in human macrophage-like cells derived from the monocytic cell line THP-1. The gene expression of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-12 (p40), IL-10 and GAPDH in macrophages treated with PM, TM and DM at steady and activated (inflammatory) states were measured using real-time reverse transcription-polymerase chain reaction. TNF-α and IL-6 in macrophages (both states) with DM were higher than PM or TM. IL-10 in steady state macrophages with DM was higher than PM whereas DM increased IL-10 in activated macrophages compared with TM. TM increased IL-6 and IL-12 (p40) in steady state macrophages compared with PM. IL-12 (p40) in activated macrophages with TM was higher than PM. IL-10 in steady state macrophages with TM was higher than PM. These results suggest that DM induces higher gene expression of pro-inflammatory and anti-inflammatory cytokines in macrophages compared with PM or TM. PM reduced gene expression of pro-inflammatory cytokines compared with TM, which may decrease the development of necrotizing enterocolitis and systematic inflammation. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://www.mdpi.com/1420-3049/25/10/2376; https://doaj.org/toc/1420-3049; https://doaj.org/article/30a8b6a54ac84c58a2397109c34d7f53 |
| DOI: |
10.3390/molecules25102376 |
| Availability: |
https://doi.org/10.3390/molecules25102376; https://doaj.org/article/30a8b6a54ac84c58a2397109c34d7f53 |
| Accession Number: |
edsbas.B2C6D83A |
| Database: |
BASE |