| Title: |
Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification |
| Authors: |
Rodriguez Temporal, David; Alcaide Fernández de Vega, Fernando; Mareković, Ivana; O’Connor, James Anthony; Gorton, Rebecca; Ingen, Jakko van; Bossche, An van den; Héry-Arnaud, Genevieve; Beauruelle, Clémence; Orth-Höller, Dorothea; Palacios Gutiérrez, Juan José; Tudó i Vilanova, Griselda; Bou, Germán; Ceyssens, Pieter-Jan; Garrigó, Montserrat; González Martín, Julián; Greub, Gilbert; Hrabak, Jaroslav; Ingebretsen, André; Mediavilla Gradolph, Maria Concepción; Oviaño, Marina; Palop, Begoña; Pranada, Arthur B.; Quiroga, Lidia; Ruiz Serrano, Maria Jesús; Rodríguez Sánchez, Belén |
| Source: |
Articles publicats en revistes (Fonaments Clínics) |
| Publisher Information: |
Springer Science and Business Media LLC |
| Publication Year: |
2022 |
| Collection: |
Dipòsit Digital de la Universitat de Barcelona |
| Subject Terms: |
Malalties del pulmó; Bacteris patògens; Pulmonary diseases; Pathogenic bacteria |
| Description: |
The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Lowenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score >= 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification. |
| Document Type: |
article in journal/newspaper |
| File Description: |
7 p.; application/pdf |
| Language: |
English |
| Relation: |
Reproducció del document publicat a: https://doi.org/10.1038/s41598-022-05315-7; Scientific Reports, 2022, vol 12, num 1; https://doi.org/10.1038/s41598-022-05315-7; https://hdl.handle.net/2445/183115 |
| Availability: |
https://hdl.handle.net/2445/183115 |
| Rights: |
cc by (c) Rodriguez Temporal, David et al, 2022 ; http://creativecommons.org/licenses/by/3.0/es/ ; info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.B4259F11 |
| Database: |
BASE |