| Title: |
People with obesity exhibit losses in muscle proteostasis that are partly improved by exercise training |
| Authors: |
Srisawat, K; Stead, CA; Hesketh, K; Pogson, MA; Strauss, JA; Cocks, MS; Siekmann, I; Phillips, SM; Lisboa, PJ; Shepherd, SO; Burniston, JG |
| Publisher Information: |
Wiley |
| Publication Year: |
2023 |
| Collection: |
Liverpool John Moores University: LJMU Research Online |
| Subject Terms: |
RA0421 Public health. Hygiene. Preventive Medicine; RC1200 Sports Medicine |
| Description: |
This pilot experiment examines if a loss in muscle proteostasis occurs in people with obesity and whether endurance exercise positively influences either the abundance profile or turnover rate of proteins in this population. Men with (n = 3) or without (n = 4) obesity were recruited and underwent a 14-d measurement protocol of daily deuterium oxide (D2 O) consumption and serial biopsies of vastus lateralis muscle. Men with obesity then completed 10-weeks of high-intensity interval training (HIIT), encompassing 3 sessions per week of cycle ergometer exercise with 1 min intervals at 100% maximum aerobic power interspersed by 1 min recovery periods. The number of intervals per session progressed from 4 to 8, and during weeks 8-10 the 14-d measurement protocol was repeated. Proteomic analysis detected 352 differences (p < 0.05, false discovery rate < 5%) in protein abundance and 19 (p < 0.05) differences in protein turnover, including components of the ubiquitin-proteasome system. HIIT altered the abundance of 53 proteins and increased the turnover rate of 22 proteins (p < 0.05) and tended to benefit proteostasis by increasing muscle protein turnover rates. Obesity and insulin resistance are associated with compromised muscle proteostasis, which may be partially restored by endurance exercise. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text |
| Language: |
English |
| ISSN: |
1615-9853 |
| Relation: |
https://researchonline.ljmu.ac.uk/id/eprint/21904/1/People%20with%20obesity%20exhibit%20losses%20in%20muscle%20proteostasis%20that%20are%20partly%20improved%20by%20exercise%20training.pdf; Srisawat, K, Stead, CA, Hesketh, K, Pogson, MA, Strauss, JA ORCID logoorcid:0000-0001-7175-2494 , Cocks, MS, Siekmann, I ORCID logoorcid:0000-0002-0706-6307 , Phillips, SM, Lisboa, PJ ORCID logoorcid:0000-0001-6365-4499 , Shepherd, SO and Burniston, JG ORCID logoorcid:0000-0001-7303-9318 (2023) People with obesity exhibit losses in muscle proteostasis that are partly improved by exercise training. Proteomics. pp. 1-14. ISSN 1615-9853 |
| DOI: |
10.1002/pmic.202300395 |
| Availability: |
https://researchonline.ljmu.ac.uk/id/eprint/21904/; https://researchonline.ljmu.ac.uk/id/eprint/21904/1/People%20with%20obesity%20exhibit%20losses%20in%20muscle%20proteostasis%20that%20are%20partly%20improved%20by%20exercise%20training.pdf; https://doi.org/10.1002/pmic.202300395 |
| Rights: |
cc_by |
| Accession Number: |
edsbas.B44F4B8C |
| Database: |
BASE |