| Title: |
The vertebrate Aqp14 water channel is a neuropeptide-regulated polytransporter |
| Authors: |
Chauvigné, François; Yilmaz, Ozlem; Ferré, Alba; Fjelldal, Per Gunnar; Finn, Roderick Nigel; Cerdà, Joan |
| Publication Year: |
2019 |
| Collection: |
Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB |
| Subject Terms: |
Evolutionary biology; Ichthyology; Molecular biology |
| Description: |
Water channels (aquaporins) were originally discovered in mammals with fourteen subfamilies now identified (AQP0-13). Here we show that a functional Aqp14 subfamily phylogenetically related to AQP4-type channels exists in all vertebrate lineages except hagfishes and eutherian mammals. In contrast to the water-selective classical aquaporins, which have four aromatic-arginine constriction residues, Aqp14 proteins present five non-aromatic constriction residues and facilitate the permeation of water, urea, ammonia, HO and glycerol. Immunocytochemical assays suggest that Aqp14 channels play important osmoregulatory roles in piscine seawater adaptation. Our data indicate that Aqp14 intracellular trafficking is tightly regulated by the vasotocinergic/isotocinergic neuropeptide and receptor systems, whereby protein kinase C and A transduction pathways phosphorylate highly conserved C-terminal residues to control channel plasma membrane insertion. The neuropeptide regulation of Aqp14 channels thus predates the vasotocin/vasopressin regulation of AQP2-5-6 orthologs observed in tetrapods. These findings demonstrate that vertebrate Aqp14 channels represent an ancient subfamily of neuropeptide-regulated polytransporters. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISSN: |
23993642; 00050237 |
| Relation: |
Agencia Estatal de Investigación AGL2016-76802-R; Ministerio de Economía y Competitividad RYC-2015-17103; Ministerio de Economía y Competitividad BES-2014-068745; Communications Biology; Vol. 2 (2019), art. 462; https://ddd.uab.cat/record/222923; urn:oai:ddd.uab.cat:222923; urn:pmid:31840107; urn:scopus_id:85076425280; urn:articleid:23993642v2e462; urn:wos_id:000502375900001; urn:altmetric_id:72622132; urn:pmcid:PMC6906440; urn:oai:pubmedcentral.nih.gov:6906440 |
| Availability: |
https://ddd.uab.cat/record/222923 |
| Rights: |
open access ; Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. ; https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.B4934AA5 |
| Database: |
BASE |