| Title: |
Towards standardization of immune functional assays |
| Authors: |
Mouton, William; Albert-Vega, Chloé; Boccard, Mathilde; Bartolo, François; Oriol, Guy; Lopez, Jonathan; Pachot, Alexandre; Textoris, Julien; Mallet, François; Brengel-Pesce, Karen; Trouillet-Assant, Sophie |
| Contributors: |
BIOMERIEUX Lyon, France; Virology and human respiratory Pathologies - Virology and human respiratory Pathologies CIRI (CIRI-VirPath); Centre International de Recherche en Infectiologie (CIRI); École normale supérieure de Lyon (ENS de Lyon); Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon); Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS); Université Claude Bernard Lyon 1 (UCBL); Université de Lyon; Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL); Centre Léon Bérard Lyon -Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS); Hospices Civils de Lyon (HCL); Physiopathologie de l'immunodépression associée aux réponses inflammatoires systémiques / Pathophysiology of Injury-induced Immunosuppression (PI3); Université de Lyon-Université de Lyon |
| Source: |
ISSN: 1521-6616. |
| Publisher Information: |
CCSD; Elsevier |
| Publication Year: |
2020 |
| Collection: |
Université Jean Monnet – Saint-Etienne: HAL |
| Subject Terms: |
Host response; Stimulation; Standardization; Independent validation; Immune functional assay; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; Recent advances in the immunotherapy field require evaluation of the immune function to adapt therapeutic decisions. Immune functional assays (IFA) are able to reveal the immune status and would be useful to further adapt and/or improve patient's care. However, standardized methods are needed to implement IFA in clinical settings. We carried out an independent validation of a published method used to characterize the underlying host response to infectious conditions using an IFA. We evaluated the reproducibility and robustness of this IFA and the associated readout using an independent healthy volunteers (HV) cohort. Expression of a 44-gene signature and IFNγ protein secretion was assessed after stimulation. We observed a strong host-response correlation between the two cohorts. We also highlight that standardized methods for immune function evaluation exist and could be implemented in larger-scale studies. This IFA could be a relevant tool to reveal innate and adaptive immune dysfunction in immune-related disorders patients. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/31760096; BIORXIV: 718627; PUBMED: 31760096 |
| DOI: |
10.1016/j.clim.2019.108312 |
| Availability: |
https://hal.science/hal-03489061; https://hal.science/hal-03489061v1/document; https://hal.science/hal-03489061v1/file/S152166161930405X.pdf; https://doi.org/10.1016/j.clim.2019.108312 |
| Rights: |
http://creativecommons.org/licenses/by-nc/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.B507EC00 |
| Database: |
BASE |