| Title: |
Stability of immobilized horseradish peroxidase in 1 water-miscible organic solvents |
| Authors: |
Arshi, Simin; Tasker, Paul; Hailo, Goran; Curtin, Teresa; Magnar, Edmond |
| Publisher Information: |
American Chemical Society |
| Publication Year: |
2026 |
| Collection: |
University of Limerick: Institutional Repository (ULIR) |
| Subject Terms: |
chitosan beads; phytic acid; enzyme; organic solvents; immobilization |
| Description: |
Chitosan beads were prepared with phytic acid to develop a biocompatible matrix for 8 enzyme immobilization. Horseradish peroxidase (HRP) was immobilized on phytic acid modified 9 chitosan beads and its catalytic activity measured using 2-methoxyphenol. The concentration of 10 phytic acid used was varied, with optimal enzyme activity at a concentration of 25 mM phytic 11 acid. The phytic acid/chitosan beads were characterized using FTIR, SEM, and their swelling 12 behavior was investigated at room temperature. The beads exhibited slightly higher levels of water 13 absorption, 20±2 of mass for phytic acid phytic acid/chitosan versus 18.6±1.5 for chitosan beads. 14 The activity of HRP (free and immobilized) was examined in aqueous-nonaqueous mixtures (5:95) 15 of dimethylformamide (DMF), hydroxymethylfurfural (HMF), methanol (MeOH), acetonitrile 16 (ACN), ethanol (EtOH), acetone, and propanol. Immobilized HRP showed good stability in DMF, 17 MeOH, ACN, EtOH, and acetone, maintaining over 40% of its initial activity after 6 h incubation in the solvent mixtures, demonstrating the successful use of phytic acid-chitosan as an enzyme 1 support. ; Research Ireland Centre for Pharmaceuticals (SSPC) , the Irish Research Council (IRC) (P.T.), and the Faculty of Science and Engineering (summer bursary to G.H.) ; 12/RC/2275 ; GOIPG/2022/1899 |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
Langmuir; https://hdl.handle.net/10344/31577; https://doi.org/10.34961/19216 |
| DOI: |
10.34961/19216 |
| DOI: |
10.1021/acs.langmuir.5c04417 |
| Availability: |
https://hdl.handle.net/10344/31577; https://doi.org/10.34961/19216; https://doi.org/10.1021/acs.langmuir.5c04417 |
| Rights: |
Attribution-NonCommercial-ShareAlike 4.0 International ; http://creativecommons.org/licenses/by-nc-sa/4.0/ ; This document is the Accepted Manuscript version of a Published Work that appeared in final form in Langmuir, copyright © 2025 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.langmuir.5c04417. |
| Accession Number: |
edsbas.B6F72BE7 |
| Database: |
BASE |