| Title: |
A high-quality reference genome for the fission yeast Schizosaccharomyces osmophilus [preprint] |
| Authors: |
Jia, Guo-Song; Zhang, Wen-Cai; Liang, Yue; Liu, Xi-Han; Rhind, Nicholas; Pidoux, Alison; Brysch-Herzberg, Michael; Du, Li-Lin |
| Contributors: |
Biochemistry and Molecular Biotechnology |
| Source: |
bioRxiv |
| Publisher Information: |
Cold Spring Harbor Laboratory |
| Publication Year: |
2022 |
| Collection: |
University of Massachusetts, Medical School: eScholarship@UMMS |
| Subject Terms: |
Genomics; fission yeast; genome sequencing |
| Description: |
This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review. The PDF available for download is Version 2 of this preprint. The complete version history of this preprint is available at https://doi.org/10.1101/2022.12.06.519325. ; Fission yeasts are an ancient group of fungal species that diverged from each other from tens to hundreds of million years ago. Among them is the preeminent model organism Schizosaccharomyces pombe, which has significantly contributed to our understandings of molecular mechanisms underlying fundamental cellular processes. The availability of the genomes of S. pombe and three other fission yeast species S. japonicus, S. octosporus, and S. cryophilus has enabled cross-species comparisons that provide insights into the evolution of genes, pathways, and genomes. Here, we performed genome sequencing on the type strain of the recently identified fission yeast species S. osmophilus and obtained a complete mitochondrial genome and a nuclear genome assembly with gaps only at rRNA gene arrays. A total of 5098 protein-coding nuclear genes were annotated and orthologs for more than 95% of them were identified. Genome-based phylogenetic analysis showed that S. osmophilus is most closely related to S. octosporus and these two species diverged around 16 million years ago. To demonstrate the utility of this S. osmophilus reference genome, we conducted cross-species comparative analyses of centromeres, telomeres, transposons, the mating-type region, Cbp1 family proteins, and mitochondrial genomes. These analyses revealed conservation of repeat arrangements and sequence motifs in centromere cores, identified telomeric sequences composed of two types of repeats, delineated relationships among Tf1/sushi group retrotransposons, characterized the evolutionary origins and trajectories of Cbp1 family domesticated transposases, and discovered signs of interspecific transfer of two types of mitochondrial selfish elements. |
| Document Type: |
report |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
Now published in G3 Genes%7CGenomes%7CGenetics doi:10.1093/g3journal/jkad028; bioRxiv; https://doi.org/10.1101/2022.12.06.519325; http://hdl.handle.net/20.500.14038/51487 |
| DOI: |
10.1101/2022.12.06.519325 |
| Availability: |
https://doi.org/10.1101/2022.12.06.519325; https://hdl.handle.net/20.500.14038/51487 |
| Rights: |
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.; Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.B72D991D |
| Database: |
BASE |