Cytoplasmic Juxtamembrane Region of the Insulin Receptor: A Critical Role in ATP Binding, Endogenous Substrate Phosphorylation, and Insulin-Stimulated Bioeffects in CHO Cellst
| Title: | Cytoplasmic Juxtamembrane Region of the Insulin Receptor: A Critical Role in ATP Binding, Endogenous Substrate Phosphorylation, and Insulin-Stimulated Bioeffects in CHO Cellst |
|---|---|
| Authors: | Jonathan M. Backer; Gregory G. Schroeder; T Deborah A. Cahil; T Axel Ullrich; L Kenneth Siddle; Morris F. White |
| Contributors: | The Pennsylvania State University CiteSeerX Archives |
| Source: | http://www.whitelabs.org/publications/pdf/1991/MFW61.pdf. |
| Publication Year: | 1990 |
| Collection: | CiteSeerX |
| Description: | We have expressed in C H O cells a mutant receptor (IRA960) from which 12 amino acids in the juxtamembrane region (A954-D965), including Tyrm have been deleted. The mutant receptor bound insulin normally but exhibited an increased K,,, for ATP during autophosphorylation. Upon prolonged incubation in vitro, or at high ATP concentrations such as those observed in vivo, autophosphorylation of IRA960 was similar to wild type, and the in vitro phosphotransferase activity of the autophosphorylated IRAW was normal. These results suggest that the deletion did not cause a nonspecific structural disruption of the catalytic domain |
| Document Type: | text |
| File Description: | application/pdf |
| Language: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.501.2892; http://www.whitelabs.org/publications/pdf/1991/MFW61.pdf |
| Availability: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.501.2892; http://www.whitelabs.org/publications/pdf/1991/MFW61.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| Accession Number: | edsbas.B72E7671 |
| Database: | BASE |