| Title: |
Hepatotropic Peptides Grafted onto Maleimide-Decorated Nanoparticles: Preparation, Characterization and In Vitro Uptake by Human HepaRG Hepatoma Cells |
| Authors: |
Brossard, Clarisse; Vlach, Manuel; Jacquet, L; Vene, Elise; Dorcet, Vincent; Loyer, Pascal; Cammas-Marion, Sandrine; Lepareur, Nicolas |
| Contributors: |
Institut des Sciences Chimiques de Rennes (ISCR); Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes); Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS); Synthèse Caractérisation Analyse de la Matière (ScanMAT); Université de Rennes (UR)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS); Nutrition, Métabolismes et Cancer (NuMeCan); Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage Rennes (PEGASE); Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro); Centre Hospitalier Universitaire de Rennes CHU Rennes = Rennes University Hospital Pontchaillou; CRLCC Eugène Marquis (CRLCC); UNICANCER; This work was funded by the Fondation pour la Recherche Médicale (FRM N°DCM20181039540), Cancéropôle Grand Ouest (CGO n° 17/028), Labex IRON (n° ANR-11-LABX-0018), Institut National de la Santé et de la Recherche Médicale (Inserm), l’Ecole Nationale Supérieure de Chimie de Rennes (ENSCR, France), the Centre National de la Recherche Scientifique (CNRS). Clarisse Brossard was recipient of a PhD fellowship from the Fondation pour la Recherche Médicale (FRM N°DCM20181039540) and Elise Vène received a 3-year PhD fellowship from the Association pour la Recherche contre le Cancer (ARC, France).; ANR-11-LABX-0018,IRON,Radiopharmaceutiques Innovants en Oncologie et Neurologie(2011) |
| Source: |
ISSN: 2073-4360 ; Polymers ; https://hal.science/hal-03716595 ; Polymers, 2022, 14 (12), pp.2447. ⟨10.3390/polym14122447⟩. |
| Publisher Information: |
HAL CCSD; MDPI |
| Publication Year: |
2022 |
| Collection: |
Université de Rennes 1: Publications scientifiques (HAL) |
| Subject Terms: |
HepaRG cells; biocompatible NPs; hepatotropic peptides; poly(benzyl malate); post-formulation modification; [CHIM]Chemical Sciences |
| Description: |
International audience ; We recently demonstrated the strong tropism of George Baker (GB) Virus A (GBVA10-9) and protein (CPB) derived synthetic peptides towards hepatoma cells. In a first approach, these peptides were covalently bound to poly(benzyl malate) (PMLABe) and poly(ethylene glycol)--PMLABe (PEG--PMLABe) (co)polymers, and corresponding peptide-decorated nanoparticles (NPs) were prepared by nanoprecipitation. We showed that peptide enhanced NPs internalization by hepatoma cells. In the present work, we set up a second strategy to functionalize NPs prepared from PMLABe derivates. First, maleimide-functionalized PMLABe (Mal-PMLABe) and PEG--PMLABe (Mal-PEG--PMLABe) were synthesized and corresponding NPs were prepared by nanoprecipitation. Then, peptides (GBVA10-9, CPB and their scramble controls GBVA10-9scr and CPBscr) with a thiol group were engrafted onto the NPs' maleimide groups using the Michael addition to obtain peptide functionalized NPs by post-formulation procedure. These peptide-modified NPs varied in diameter and dispersity depending on the considered peptides and/or (co)polymers but kept their spherical shape. The peptide-functionalized NPs were more efficiently internalized by HepaRG hepatoma cells than native and maleimide-NPs with various levels relying on the peptide's nature and the presence of PEG. We also observed important differences in internalization of NPs functionalized by the maleimide-thiol-peptide reaction compared to that of NPs prepared from peptide-functionalized PMLABe derivatives. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/35746020; PUBMED: 35746020; WOS: 000916170600001 |
| DOI: |
10.3390/polym14122447 |
| Availability: |
https://hal.science/hal-03716595; https://hal.science/hal-03716595v1/document; https://hal.science/hal-03716595v1/file/polymers-14-02447.pdf; https://doi.org/10.3390/polym14122447 |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.B7ED6877 |
| Database: |
BASE |