| Description: |
Wild carrot has been used in traditional medicine in Lebanon to treat several diseases. Recently, Daucus carota oil extract and its fractions, F1 (pentane; 100%), F2 (pentane-diethyl ether; 50:50), F3 (diethyl ether; 100%) and F4 (chloroform-methanol; 93:7), were shown to possess anticancer and antioxidant activities [1,2]. The present study aims to complete spectrum of the anticancer activity of the 4 fractions against three types of human epidermal keratinocytes (HaCaT cells) cell lines HaCaT-II4 (non-invasive), HaCatT-A5 (invasive) and HaCaT (immortalized). Cells were treated with 10, 25, 50 and 100 µg/mL of the fractions for 48h and cell survival was determined using WST-1 assay. The 4 fractions exhibited a dose-dependent inhibition of cell viability, with F1 and F2 being more potent (p < 0.05) than F3 and F4. The immortalized HaCat cell line was more resistant to cytotoxicity compared with the two malignant counterparts (p < 0.05). The IC50 values of F1, F2, F3, and F4 fractions in the immortalized HaCat cells were respectively 34, 37, 50 and 45 µg/mL, which were significantly (p < 0.05) higher than that of HaCat-A5 cells with IC50 values of 26, 32, 38 and 36 µg/mL respectively and of HaCat-II4 cells with IC50 values of 26, 24, 39 and 34 µg/mL. In conclusion, the results show that F1 and F2 are more toxic to invasive and non-invasive HaCaT than the immortalized cells. Further studies are needed to isolate and characterize the biologically active compounds in F1 and F2. ; Published ; N/A |