| Title: |
INFLUENCE OF IRON ON GUT MICROBIAL COMPOSITION IN ACUTE DSS-INDUCED COLITIS IN C57BL/6 MICE |
| Authors: |
Mahalhal, A; Campbell, B; Pritchard, DM; Probert, C |
| Publisher Information: |
BMJ Publishing Group Ltd and British Society of Gastroenterology |
| Publication Year: |
2017 |
| Collection: |
The University of Liverpool Repository |
| Description: |
Iron deficiency anaemia is common in inflammatory bowel disease (IBD). Iron supplementation may induce or exacerbate colitis in rats (APT 2001; 15:1989–99). Dysbiosis is common in IBD and iron contributes to this as it is a growth factor for some bacteria. We investigated the acute effect of dietary iron supplementation and/or reduction on the intestinal microbiome in a murine model of colitis. We report results of changes at the phylum level. Studies were performed on six groups of wild-type mice (6/group). Acute colitis was induced with 2% dextran sodium sulphate (DSS) for 5 days, followed by 5 further days on water. DSS-treated mice were fed one of three diets (from day-1 of DSS treatment): Low iron [LI] (100ppm), normal iron [NI] (200ppm) and high iron [HI] supplemented (400ppm) chow. Also, three non-DSS-treated groups were studied and fed similarly. Clinical and pathological data were compared at day-1 vs. day-10, bacterial gDNA was extracted from faeces and microbiota composition determined from the sequence of V4 region of 16 S rDNA on the Illumina MiSeq platform. Statistical inferences were made using Welch’s t-test with post-hoc analysis (Bioinformatics 2010; 26:715–21). DSS-induced colitis was exacerbated in LI and HI groups, more so in LI mice (as determined by mean total body weight loss [9.2%, at day-8] and significantly worse histology; median score 2.5, at day-10). However, faecal phyla changes were only seen following DSS treatment in the HI group at day-10 vs. day-1 with increased Proteobacteria (p |
| Document Type: |
conference object |
| File Description: |
text |
| Language: |
English |
| Relation: |
https://livrepository.liverpool.ac.uk/3027459/1/BSG%202017%20%281%29%20final%20draft.pdf; Mahalhal, A, Campbell, B orcid:0000-0002-7407-012X , Pritchard, DM orcid:0000-0001-7971-3561 and Probert, C orcid:0000-0003-0477-6714 (2017) INFLUENCE OF IRON ON GUT MICROBIAL COMPOSITION IN ACUTE DSS-INDUCED COLITIS IN C57BL/6 MICE In: British Society of Gastroenterology, Annual General Meeting, 19–22 June 2017, Abstracts. |
| DOI: |
10.1136/gutjnl-2017-314472.456 |
| Availability: |
https://livrepository.liverpool.ac.uk/3027459/; https://doi.org/10.1136/gutjnl-2017-314472.456; https://livrepository.liverpool.ac.uk/3027459/1/BSG%202017%20%281%29%20final%20draft.pdf |
| Accession Number: |
edsbas.B90D3F9F |
| Database: |
BASE |