| Title: |
Cell-intrinsic and -extrinsic functions of the ESCRT-III component shrub in cytokinetic abscission of Drosophila sensory organ precursor |
| Authors: |
Bruelle, Céline; Pinot, Mathieu; Daniel, Emeline; Daudé, Marion; Mathieu, Juliette; Le Borgne, Roland |
| Contributors: |
Institut de Génétique et Développement de Rennes (IGDR); Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes (Biosit : Biologie - Santé - Innovation Technologique); Centre interdisciplinaire de recherche en biologie (CIRB); Labex MemoLife; École normale supérieure - Paris (ENS-PSL); Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris); Université Paris Sciences et Lettres (PSL)-École normale supérieure - Paris (ENS-PSL); Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS); Ligue contre le Cancer; Equipe Labellisée; ANR-16-CE13-004-01, Agence Nationale de la Recherche; ANR-16-CE13-0004,CYTOSIGN,Polarité épithéliale et signalisation au cours de la cytodiérèse des cellules animales(2016); ANR-20-CE13-0015,ACTRICE,Contribution des Jonctions Tricellulaires dans la Mécanique Epithéliale(2020) |
| Source: |
ISSN: 0950-1991. |
| Publisher Information: |
HAL CCSD; Company of Biologists |
| Publication Year: |
2023 |
| Collection: |
Université de Rennes 1: Publications scientifiques (HAL) |
| Subject Terms: |
Asymmetric division; Cytokinesis; ESCRT-III; Epithelium; Septate junctions; [SDV.BDD]Life Sciences [q-bio]/Development Biology; [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] |
| Description: |
International audience ; Although the molecular mechanisms governing abscission of isolated cells have largely been elucidated, those underlying the abscission of epithelial progenitors surrounded by epidermal cells (ECs), connected via cellular junctions, remain largely unexplored. Here, we investigated the remodeling of the paracellular diffusion barrier ensured by septate junctions (SJs) during cytokinesis of Drosophila sensory organ precursors (SOPs). We found that SOP cytokinesis involves the coordinated, polarized assembly and remodeling of SJs in the dividing cell and its neighbors, which remain connected to the former via membrane protrusions pointing towards the SOP midbody. SJ assembly and midbody basal displacement occur faster in SOPs than in ECs, leading to quicker disentanglement of neighboring cell membrane protrusions prior to midbody release. As reported in isolated cells, the endosomal sorting complex required for the transport-III component Shrub/CHMP4B is recruited at the midbody and cell-autonomously regulates abscission. In addition, Shrub is recruited to membrane protrusions and is required for SJ integrity, and alteration of SJ integrity leads to premature abscission. Our study uncovers cell-intrinsic and -extrinsic functions of Shrub in coordinating remodeling of the SJs and SOP abscission. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/37226981; PUBMED: 37226981 |
| DOI: |
10.1242/dev.201409 |
| Availability: |
https://hal.science/hal-04100234; https://hal.science/hal-04100234v1/document; https://hal.science/hal-04100234v1/file/2023,%20Bruelle_Mathieu_Leborgne_dev.pdf; https://doi.org/10.1242/dev.201409 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.B992B2D3 |
| Database: |
BASE |