| Title: |
Interaction of human gut microbiota and local immune system in progression of colorectal adenoma (MIMICA-1): a protocol for a prospective, observational cohort study / |
| Authors: |
Valčiukienė, Jūratė; Lastauskienė, Eglė; Laurinavičienė, Aida; Jakubauskas, Matas; Kryžauskas, Marius; Valkiūnienė, Rūta Barbora; Augulis, Renaldas; Garnelytė, Aušra; Kavoliūnas, Justinas; Šilinskaitė, Ugnė; Poškus, Tomas |
| Source: |
Frontiers in oncology., Lausanne : Frontiers Media SA, 2024, vol. 14, p. [1-13]. ; eISSN 2234-943X |
| Publication Year: |
2025 |
| Collection: |
Vilnius University Virtual Library (VU VL) / Vilniaus universitetas virtuali biblioteka |
| Subject Terms: |
adenocarcinoma; bacterial dysbiosis; carcinogenesis; carcinoma in situ; colorectal adenoma; colorectal polyps; gut microbiota; intestinal immunity |
| Description: |
Introduction: The current understanding of colorectal carcinogenesis is based on the adenoma-carcinoma sequence, where genetics, intestinal microbiota changes and local immunity shifts seem to play the key roles. Despite the emerging evidence of dysbiotic intestinal state and immune-cell infiltration changes in patients with colorectal adenocarcinoma, early and advanced adenoma as precursors of colorectal cancer, and carcinoma in situ as the following progression, are rather less studied. The newly colon-site adapted AI-based analysis of immune infiltrates is able to predict long-term outcomes of colon carcinoma. Though it could also facilitate the pathologic evaluation of precancerous lesion’s potential to progress. Therefore, the purpose of this prospective cohort study (MIMICA-1) is, firstly, to identify the intestinal microbiota and immune infiltration patterns around the normal bowel tissue, early and advanced adenoma, carcinoma in situ, and adenocarcinoma, and secondly, to analyze the immune – microbiome interplay along the steps of conventional colorectal tumorigenesis. Methods and analyses: This study aims to prospectively recruit 40 patients (10 per group) with confirmed colorectal dysplasia undergoing endoscopic polypectomy, endoscopic mucosal resection for colorectal small (≤1cm), and large (>1cm) adenoma or carcinoma in situ, or biopsy and subsequent colon resection for invasive colorectal cancer, and 10 healthy patients undergoing screening colonoscopy. Stool samples will be collected prior to bowel preparation for the analysis of fecal (luminal) microbiota composition. Biopsy specimens will be taken from the terminal ileum, right colon, left colon, and a pathological lesion in the colon (if present) to assess mucosa-associated microbiota composition and intestinal immunity response. DNA will be extracted from all samples and sequenced using the Illumina MiSeq platform. Unifrac and Bray-Curtis methods will be used to assess microbial diversity. The intestinal immune system response will be ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
https://epublications.vu.lt/object/elaba:222744783/222744783.pdf; https://repository.vu.lt/VU:ELABAPDB222744783&prefLang=en_US |
| Availability: |
https://repository.vu.lt/VU:ELABAPDB222744783&prefLang=en_US |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.B9D2D8DE |
| Database: |
BASE |