| Title: |
Functional Topography in the Rat Rostral Ventrolateral Medulla (RVLM): Distribution of C1 Neurons that Respond to Cardiovascular versus Metabolic Stimuli |
| Authors: |
Llewellyn‐Smith, Ida J; Senthilkumaran, Manjula; Spradley, Frank; Etelvino, Gisele M; Bobrovskaya, Larisa; Carrive, Pascal; Mueller, Patrick J; Verberne, Anthony J M |
| Contributors: |
National Health and Medical Research Council; National Institutes of Health |
| Source: |
The FASEB Journal ; volume 33, issue S1 ; ISSN 0892-6638 1530-6860 |
| Publisher Information: |
Wiley |
| Publication Year: |
2019 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Neurons controlling cardiovascular responses and glucose counter regulation are co‐located within the RVLM, with activated neurons lying almost exclusively caudal to the caudal pole of the facial nucleus (FN). However, it is unclear whether the same neurons are involved in triggering both cardiovascular and metabolic responses. We compared the distributions of RVLM C1 neurons activated by cardiovascular or metabolic stimuli using Fos to identify activated neurons. To conscious male or female rats, we administered insulin (10 U/kg ip) or 2‐deoxyglucose (2DG; 400 mg/kg sc) to induce hypoglycaemia or nitroprusside (NP; 1 mg/ml iv), hydralazine (HDZ; 10 mg/kg iv) or diazoxide (DZX; 50 mg/kg iv) to cause hypotension. Rats were transcardially perfused with formaldehyde 90 or 120 minutes later. Coronal 30‐micrometer thick sections of medulla (1:4 series) from the spinomedullary junction to mid‐FN were immunoperoxidase‐stained to show Fos‐immunoreactivity plus phenylethanolamine N‐methyl transferase (PNMT)‐immunoreactivity to identify C1 neurons. The 6 sections containing the 600 micrometers caudal to the caudal pole of FN (sections FN0 to FN‐5) were each divided into 3 equal segments (medial, middle, lateral) and the total numbers of PNMT neurons with Fos nuclei (Fos+PNMT neurons) were counted. The numbers of Fos+PNMT neurons were greater in the medial and middle thirds than in the lateral third of the C1 cell column in insulin‐ and 2DG‐treated rats. In NP‐, DZX‐or HDZ‐treated rats, the numbers of Fos+PNMT neurons were greater in the middle and lateral two thirds of the C1 cell column. Two‐way ANOVA showed a very strong interaction between stimulus and laterality (p |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1096/fasebj.2019.33.1_supplement.742.8 |
| Availability: |
http://dx.doi.org/10.1096/fasebj.2019.33.1_supplement.742.8 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.B9F1B0F9 |
| Database: |
BASE |