Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence
| Title: | Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence |
|---|---|
| Authors: | Liu, Chen; Malek, Michael; Poon, Ivan; Jiang, Lanzhou; Siddiquee, Arif M.; Sheppard, Colin J.R.; Roberts, Ann; Quiney, Harry; Zhang, Douguo; Yuan, Xiaocong; Lin, Jiao; Depeursinge, Christian; Marquet, Pierre; Kou, Shan Shan |
| Contributors: | Biological and Environmental Sciences and Engineering (BESE) Division; Department of Chemistry and Physics, La Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne, Victoria, 3086, Australia; Department of Optics and Optical Engineering, Anhui Key Laboratory of Optoelectronic Science and Technology, University of Science and Technology of China, Hefei, 230026, China; Nanophotonics Research Centre, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Shenzhen University, Shenzhen, 518060, China; Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne, Victoria, 3086, Australia; Australian Research Council (ARC), Centre of Excellence in Advanced Molecular Imaging, Australia; Istituto Italiano di Tecnologia, Genova, 16163, Italy; School of Physics, University of Melbourne, Victoria, 3010, Australia; School of Engineering, RMIT University, Melbourne, Victoria, 3001, Australia; Microvision and Microdiagnostic Group (SCI STI CHD), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland; Joint International Research Unit in Neurodevelopment and Child Psychiatry, CHUV, Département de Psychiatrie, Lausanne, Switzerland; Institut universitaire en santé mentale de Québec, Québec, Canada; Centre d’optique, photonique et laser, Department of Psychiatry and Neuroscience, Université Laval, 2375 rue de la Terrasse, Québec, QC, G1V 0A6, Canada |
| Publisher Information: | The Optical Society |
| Publication Year: | 2019 |
| Collection: | King Abdullah University of Science and Technology: KAUST Repository |
| Description: | We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object from the synthesized angular spectra, we obtain the quantitative subcellular morphology in refractive indices (RIs) side-by-side with its fluorescence signals. The additional contrast in RI complements the fluorescent signal, providing additional information of the targeted zones. The simultaneous dual-contrast 3D mechanism unveiled interesting information inaccessible with previous methods, as we demonstrated in the human immune cell (T cell) experiment. Further validation has been demonstrated using a Monte Carlo model. ; Australian Research Council (ARC) (DE120102352); National Natural Science Foundation of China (NSFC) (61427819); Shenzhen Science and Technology Innovation Commission (KQCS2015032416183980); Government of Guangdong Province (00201505); Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (SNF) (149652). |
| Document Type: | article in journal/newspaper |
| Language: | unknown |
| Relation: | https://www.osapublishing.org/abstract.cfm?URI=prj-7-9-1042; Liu, C., Malek, M., Poon, I., Jiang, L., Siddiquee, A. M., Sheppard, C. J. R., … Kou, S. S. (2019). Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence. Photonics Research, 7(9), 1042. doi:10.1364/prj.7.001042; Photonics Research; http://hdl.handle.net/10754/659083 |
| DOI: | 10.1364/PRJ.7.001042 |
| Availability: | http://hdl.handle.net/10754/659083; https://doi.org/10.1364/PRJ.7.001042 |
| Rights: | Archived with thanks to Photonics Research |
| Accession Number: | edsbas.BA2C22CB |
| Database: | BASE |