| Title: |
Abstract 12568: Angptl2 is Essential for Aortic Valve Development in Mice |
| Authors: |
Labbe, Pauline; Munoz Goyette, Victoria; Villeneuve, Louis; Desanlis, Ines; Alikashani, Azadeh; delwarde, constance; Thorin-Trescases, Nathalie; Kmita, Marie; Thorin, Eric |
| Source: |
Circulation ; volume 144, issue Suppl_1 ; ISSN 0009-7322 1524-4539 |
| Publisher Information: |
Ovid Technologies (Wolters Kluwer Health) |
| Publication Year: |
2021 |
| Description: |
Introduction: Angiopoietin-like 2 (Angptl2) is a pro-inflammatory anti-apoptotic protein, secreted by senescent cells. Angptl2 also contributes to maintain tissue homeostasis, and we previously reported that adult Angptl2-knockdown (KD) mice exhibit spontaneous Aortic Valve (AoV) stenosis with thickened leaflets compared to wild-type (WT) littermates. It is unknown whether and how Angptl2 contributes to AoV development. Hypothesis: Angptl2 is expressed in the AoV at the embryonic stage and is essential for its development, by contributing to programmed apoptosis/senescence and proliferation occurring during organogenesis. Methods: Embryonic hearts aged 14.5 (E14.5) and 18 days (E18) of Angptl2 -KD and WT mice were dissected. Protein expression (immunofluorescence) and leaflets/hinges thickness of AoV (hematoxylin & eosin) were quantified in histological sections. Data are mean±SEM of n embryos. Results: Compared to WT mice, in AoV leaflets from Angptl2 -KD mice at E14.5, we observed a decrease in apoptotic cells (determined by TUNEL;-47%, p |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1161/circ.144.suppl_1.12568 |
| Availability: |
https://doi.org/10.1161/circ.144.suppl_1.12568 |
| Accession Number: |
edsbas.BACEBD47 |
| Database: |
BASE |