| Title: |
Influence of binding site number for the β-estradiol-dependent induction by Z4-EV or Ste12-EV. |
| Authors: |
Sandrine Pinheiro; Mariona Nadal-Ribelles; Carme Solé; Vincent Vincenzetti; Yves Dusserre; Francesc Posas; Serge Pelet |
| Publication Year: |
2025 |
| Collection: |
The University of Auckland: Figshare |
| Subject Terms: |
Microbiology; Cell Biology; Genetics; Molecular Biology; Immunology; Developmental Biology; Science Policy; Infectious Diseases; Plant Biology; Biological Sciences not elsewhere classified; two haploid cells; saccharomyces cerevisiae < p; core promoter modulate; ste12 binding sites; binding sites; strong dimer; specific timing; largely encoded; large diversity; basal association |
| Description: |
A. Dynamics of nuclear enrichment of the dPSTR R under the control of synthetic promoters with one (blue) or two (green) Z4 binding sites (McIsaac NAR 2013) using the synthetic transcription factor Z4-EV upon stimulation with 1µm β-estradiol at time 0. B. Increase in cellular fluorescence as function of time for the reference promoter containing 6 Z4 binding sites and driving the expression of a Venus fluorescent protein which serves as an induction control for the experiment plotted in panel A. C. Dynamics of nuclear enrichment of the dPSTRR by the Ste12-EV upon stimulus by β-estradiol at time 0 under the control of different promoters containing zero (dark green), 1 PRE (light green) or two PREs in tail-to-tail orientation (blue). No detectable nuclear enrichment is observed for the 0 or 1 PRE controls, as well as, the 2 PRE spaced by 40 bp (light blue). If the 2 PREs are spaced by 3 bp (p SYN 3TT ), the induction is strong (dark blue). D. Dynamics of nuclear enrichment of the control p AGA1 -dPSTR Y by the Ste12-EV in the strains containing the synthetic promoters displayed in panel C.E. Comparison of the inducibility of p AGA1 -dPSTR Y with the Ste12 WT (solid borders) or the Ste12-EV (dashed borders). The Expression Outputs for the p AGA1 reporters induced by the Ste12 WT or the Ste12-EV were normalized relative to the Expression Output of the reference p SYN 3TT sample. The bar represents the mean response of the replicates shown by the circles. A significant difference between the normalized EO Ste12-WT and Ste12-EV is indicated by a star (t-test: p-val < 0.05) F. Fraction of responding cells for the p SYN -dPSTR R variants with the Ste12 WT (solid borders) or the Ste12-EV (dashed borders). The bar represents the mean response of the replicates shown by the circles. A significant difference between the fraction of responding cells between Ste12-WT and Ste12-EV is indicated by a star (t-test: p-val < 0.05). (PDF) |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| DOI: |
10.1371/journal.pgen.1011710.s005 |
| Availability: |
https://doi.org/10.1371/journal.pgen.1011710.s005; https://figshare.com/articles/journal_contribution/Influence_of_binding_site_number_for_the_-estradiol-dependent_induction_by_Z4-EV_or_Ste12-EV_/29332258 |
| Rights: |
CC BY 4.0 |
| Accession Number: |
edsbas.BB065A05 |
| Database: |
BASE |