| Title: |
N 6 -methyladenosine of HIV-1 RNA regulates viral infection and HIV-1 Gag protein expression |
| Authors: |
Tirumuru, Nagaraja; Zhao, Boxuan Simen; Lu, Wuxun; Lu, Zhike; He, Chuan; Wu, Li |
| Source: |
http://knowledge.uchicago.edu/record/9991. |
| Publication Year: |
2023 |
| Collection: |
Knowledge@UChicago (University of Chicago) |
| Description: |
The internal N 6 -methyladenosine (m 6 A) methylation of eukaryotic nuclear RNA controls post-transcriptional gene expression, which is regulated by methyltransferases (writers), demethylases (erasers), and m 6 A-binding proteins (readers) in cells. The YTH domain family proteins (YTHDF1-3) bind to m 6 A-modified cellular RNAs and affect RNA metabolism and processing. Here, we show that YTHDF1-3 proteins recognize m 6 A-modified HIV-1 RNA and inhibit HIV-1 infection in cell lines and primary CD4 + T-cells. We further mapped the YTHDF1-3 binding sites in HIV-1 RNA from infected cells. We found that the overexpression of YTHDF proteins in cells inhibited HIV-1 infection mainly by decreasing HIV-1 reverse transcription, while knockdown of YTHDF1-3 in cells had the opposite effects. Moreover, silencing the m 6 A writers decreased HIV-1 Gag protein expression in virus-producing cells, while silencing the m 6 A erasers increased Gag expression. Our findings suggest an important role of m 6 A modification of HIV-1 RNA in viral infection and HIV-1 protein synthesis. |
| Document Type: |
text |
| Language: |
English |
| Relation: |
https://knowledge.uchicago.edu/record/9991/files/elife-15528-v4.pdf; http://knowledge.uchicago.edu/record/9991 |
| DOI: |
10.7554/eLife.15528 |
| Availability: |
https://doi.org/10.7554/eLife.15528; http://knowledge.uchicago.edu/record/9991 |
| Accession Number: |
edsbas.BB1DB0C3 |
| Database: |
BASE |