| Title: |
SWIFT clustering analysis of intracellular cytokine staining flow cytometry data of the HVTN 105 vaccine trial reveals high frequencies of HIV-specific CD4+ T cell responses and associations with humoral responses |
| Authors: |
Tim R. Mosmann; Jonathan A. Rebhahn; Stephen C. De Rosa; Michael C. Keefer; M. Juliana McElrath; Nadine G. Rouphael; Giuseppe Pantaleo; Peter B. Gilbert; Lawrence Corey; James J. Kobie; Juilee Thakar |
| Source: |
Frontiers in Immunology, Vol 15 (2024) |
| Publisher Information: |
Frontiers Media S.A. |
| Publication Year: |
2024 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
HIV - human immunodeficiency virus; vaccine trial; reanalysis; algorithmic flow cytometry analysis; T cell response; T cell antibody correlation; Immunologic diseases. Allergy; RC581-607 |
| Description: |
IntroductionThe HVTN 105 vaccine clinical trial tested four combinations of two immunogens - the DNA vaccine DNA-HIV-PT123, and the protein vaccine AIDSVAX B/E. All combinations induced substantial antibody and CD4+ T cell responses in many participants. We have now re-examined the intracellular cytokine staining flow cytometry data using the high-resolution SWIFT clustering algorithm, which is very effective for enumerating rare populations such as antigen-responsive T cells, and also determined correlations between the antibody and T cell responses.MethodsFlow cytometry samples across all the analysis batches were registered using the swiftReg registration tool, which reduces batch variation without compromising biological variation. Registered data were clustered using the SWIFT algorithm, and cluster template competition was used to identify clusters of antigen-responsive T cells and to separate these from constitutive cytokine producing cell clusters.ResultsRegistration strongly reduced batch variation among batches analyzed across several months. This in-depth clustering analysis identified a greater proportion of responders than the original analysis. A subset of antigen-responsive clusters producing IL-21 was identified. The cytokine patterns in each vaccine group were related to the type of vaccine – protein antigens tended to induce more cells producing IL-2 but not IFN-γ, whereas DNA vaccines tended to induce more IL-2+ IFN-γ+ CD4 T cells. Several significant correlations were identified between specific antibody responses and antigen-responsive T cell clusters. The best correlations were not necessarily observed with the strongest antibody or T cell responses.ConclusionIn the complex HVTN105 dataset, alternative analysis methods increased sensitivity of the detection of antigen-specific T cells; increased the number of identified vaccine responders; identified a small IL-21-producing T cell population; and demonstrated significant correlations between specific T cell populations and serum antibody ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fimmu.2024.1347926/full; https://doaj.org/toc/1664-3224; https://doaj.org/article/52dd5afd41534ef9921ac84b943b0929 |
| DOI: |
10.3389/fimmu.2024.1347926 |
| Availability: |
https://doi.org/10.3389/fimmu.2024.1347926; https://doaj.org/article/52dd5afd41534ef9921ac84b943b0929 |
| Accession Number: |
edsbas.BD2F6AF9 |
| Database: |
BASE |