| Title: |
Impact of active lifestyle on the primary school children saliva microbiota composition |
| Authors: |
Mancini, Annamaria; Cerulli, Claudia; Vitucci, Daniela; Lasorsa, Vito Alessandro; Parente, Daniela; Di Credico, Andrea; Orrù, Stefania; Brustio, Paolo Riccardo; Lupo, Corrado; Rainoldi, Alberto; Schena, Federico; Capasso, Mario; Buono, Pasqualina |
| Contributors: |
Mancini, Annamaria; Cerulli, Claudia; Vitucci, Daniela; Lasorsa, Vito Alessandro; Parente, Daniela; Di Credico, Andrea; Orrù, Stefania; Brustio, Paolo Riccardo; Lupo, Corrado; Rainoldi, Alberto; Schena, Federico; Capasso, Mario; Buono, Pasqualina |
| Publication Year: |
2023 |
| Collection: |
Università degli Studi di Verona: Catalogo dei Prodotti della Ricerca (IRIS) |
| Subject Terms: |
children; exercise; lifestyle; microbiota; saliva |
| Description: |
Unlabelled: The aim of the study was to evaluate the effects of Active or Sedentary lifestyle on saliva microbiota composition in Italian schoolchildren. Methods: Male (114) and female children (8-10 years) belonging to five primary schools in the neighborhoods of Turin were classified as active (A) or sedentary (S) based on PAQ-C-It questionnaire. PCR amplification of salivary DNA targeted the hypervariable V3-V4 regions of the 16S rRNA bacterial genes. DADA2 workflow was used to infer the Amplicon Sequence Variants and the taxonomic assignments; the beta-diversity was obtained by PCoA with the UniFrac method; LEfSe algorithm, threshold at 5%, and Log LDA cutoff at ±0.5 were used to identify differently abundant species in A compared to S saliva sample. Daily food intake was assessed by 3-Days food record. The metabolic potential of microbial communities was assessed by PICRUSt. Results: No significant differences were found in individual's gender distribution (p = 0.411), anthropometry, BMI (p > 0.05), and all diet composition between A and S groups (p > 0.05). Eight species were differently abundant: Prevotella nigrescens (LDA score = -3.76; FDR = 1.5×10-03), Collinsella aerofaciens (LDA score = -3.17; FDR = 7.45×10-03), Simonsiella muelleri (LDA score = -2.96; FDR = 2.76×10-05), Parabacteroides merdae (LDA score = -2.43; FDR = 1.3×10-02) are enriched in the A group; Gemella parahaemolysans, Prevotella aurantiaca (LDA score = -3.9; FDR = 5.27×10-04), Prevotella pallens (LDA score = 4.23; FDR = 1.93×10-02), Neisseria mucosa (LDA score = 4.43; FDR = 1.31×10-02; LDA score = 2.94; FDR = 7.45×10-03) are enriched in the S group. A prevalence of superpathway of fatty acid biosynthesis initiation (E. coli) and catechol degradation II (meta-cleavage pathway) was found in saliva from A compared to S children. Conclusion: Our results showed that active children had an enrichment of species and genera mainly associated with a healthier profile. By contrast, the genera and the species enriched in the sedentary group ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
ELETTRONICO |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/37671197; info:eu-repo/semantics/altIdentifier/wos/WOS:001061273100001; volume:10; issue:1226891; firstpage:1; lastpage:11; numberofpages:11; journal:FRONTIERS IN NUTRITION; https://hdl.handle.net/11562/1109946; https://doi.org/10.3389/fnut.2023.1226891 |
| DOI: |
10.3389/fnut.2023.1226891 |
| Availability: |
https://hdl.handle.net/11562/1109946; https://doi.org/10.3389/fnut.2023.1226891 |
| Rights: |
info:eu-repo/semantics/openAccess ; license:Creative commons ; license uri:http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.BD9511 |
| Database: |
BASE |