| Title: |
Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits |
| Authors: |
Telliez, Cécile; Sims, Ruth; Faini, Giulia; Berto, Pascal; Papagiakoumou, Eirini; Tanese, Dimitrii; Accanto, Nicolò |
| Contributors: |
Institut de la Vision; Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS); Universität Zürich Zürich = University of Zurich (UZH); Institut universitaire de France (IUF); Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.); Université Paris Cité (UPCité); Institute for Bioengineering of Catalonia Barcelona (IBEC); National Institutes of Health (1RF1NS128772-01 and 1R01NS136027-01A1,the Institut Universitaire de France; the Region ́ Ile de France (DIM C-BRAINS); Sorbonne University (‘PARSEC’, EMRG-10/2023); the Agence National de la Recherche (PEMGet M22JRAR039); the IHU Foresight 2024 (‘HOLOCO’); European Project: 101016787,H2020-ICT-2018-20,H2020-ICT-2020-2,DEEPER(2021); European Project: 885090,ERC-2019-ADG,ERC-2019-ADG,HOLOVIS(2020); European Project: 101116227,ERC-2023-STG,ERC-2023-STG,2P-BRAINSCOPY(2024) |
| Source: |
ISSN: 2330-4022 ; ACS photonics ; https://hal.science/hal-05405471 ; ACS photonics, 2025, 12 (7), pp.3296-3318. ⟨10.1021/acsphotonics.4c02101⟩. |
| Publisher Information: |
CCSD; American Chemical Society |
| Publication Year: |
2025 |
| Subject Terms: |
Optogenetic photostimulation; Calcium and voltage imaging; Wavefront shaping; All-optical brain studies; Multiphoton microscopy; Neurophotonics; [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic; [PHYS]Physics [physics]; [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology |
| Description: |
International audience ; The possibility of using light to image and manipulate neuronal activity, at the heart of Neurophotonics, has provided new irreplaceable tools to study brain function. In particular, the combination of multiphoton microscopy and optogenetics allows researchers to interact with neuronal circuits with single-cell resolution in living brain tissues. However, significant optical challenges remain to empower new discoveries in Neuroscience. This Review focuses on three critical areas for future development: (1) expanding imaging and optogenetic stimulation to larger fields of view and faster acquisition speeds, while maintaining single-cell resolution and minimizing photodamage; (2) enabling access to deeper brain regions to study currently inaccessible neuronal circuits; and (3) developing optical techniques for studying natural behaviors in freely moving animals. For each of these challenges, we review the current state-of-the-art and suggest future directions with the potential to transform the field. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/40693197; info:eu-repo/grantAgreement//101016787/EU/DEEP BRAIN PHOTONIC TOOLS FOR CELL-TYPE SPECIFIC TARGETING OF NEURAL DISEASES/DEEPER; info:eu-repo/grantAgreement//885090/EU/Holographic control of visual circuits/HOLOVIS; info:eu-repo/grantAgreement//101116227/EU/A two-photon compound fiberscope to study the brain at all spatial and temporal scales./2P-BRAINSCOPY; PUBMED: 40693197; PUBMEDCENTRAL: PMC12279007 |
| DOI: |
10.1021/acsphotonics.4c02101 |
| Availability: |
https://hal.science/hal-05405471; https://hal.science/hal-05405471v1/document; https://hal.science/hal-05405471v1/file/multiphoton-neurophotonics-recent-advances-in-imaging-and-manipulating-neuronal-circuits.pdf; https://doi.org/10.1021/acsphotonics.4c02101 |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.BDB8ADB |
| Database: |
BASE |