| Title: |
Methodologies to assess the bioactivity of an herbal extract on immunity, health, welfare and production performance in the chicken: The case of Melissa officinalis L. extract |
| Authors: |
Travel, Angélique; Petit, Angélique; Barat, Perrine; Collin, Anne; Bourrier-Clairat, Camille; Pertusa, Marion; Skiba, Fabien; Crochet, Sabine; Cailleau-Audouin, Estelle; Chartrin, Pascal; Guillory, Vanaïque; Bellenot, Denis; Guabiraba, Rodrigo; Guilloteau, Laurence, A. |
| Contributors: |
Institut Technique de l'AVIculture (ITAVI); Biologie des Oiseaux et Aviculture (BOA - UMR 0083); Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Nutricia; Danone Nutricia Research Utrecht; Infectiologie et Santé Publique (ISP); Institut Technique Interprofessionnel des Plantes à Parfum Médicinales et Aromatiques (ITEIPMAI); INRAE; CASDAR - French Ministry of Agriculture(2016-2020 MEXAVI); CIPC (Comite Interprofessionnel du Poulet de Chair) |
| Source: |
ISSN: 2297-1769 ; Frontiers in Veterinary Science ; https://hal.inrae.fr/hal-03468715 ; Frontiers in Veterinary Science, 2021, 8, 17 p. ⟨10.3389/fvets.2021.759456⟩ ; https://www.frontiersin.org/articles/10.3389/fvets.2021.759456/full. |
| Publisher Information: |
CCSD; Frontiers Media |
| Publication Year: |
2021 |
| Collection: |
Université François-Rabelais de Tours: HAL |
| Subject Terms: |
herbal extract; Melissa officinalis; health; innate immunity; methodology; performance; poultry; welfare; [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health |
| Description: |
International audience ; The potential of herbal extracts containing bioactive compounds to strengthen immunity could contribute to reducing antimicrobial use in poultry. This study aimed at developing a reliable and robust methodological pipeline to assess the ability of herbal extracts to strengthen chicken innate defenses, especially concerning inflammation and oxidative stress. This methodology was applied to Melissa officinalis L. (MEL) extract, recognized for its biological activities including antioxidant and anti-inflammatory properties. Different methods were used to (1). guarantee the quality of MEL extract and its capacity to stimulate the innate immune system; (2). evaluate the relevance of an ex vivo model to mimic inflammatory and oxidative stress challenges to replace LPS injection in chickens; (3). analyse the effects of feed supplemented with MEL extract on inflammation and oxidative stress induced ex vivo ; (4). assess the effects of MEL extract on the redox balance, health, welfare and performance in broilers exposed to suboptimal starting conditions through a large-scale approach. The quality of MEL extract preparations, through phytochemical quantification of rosmarinic acid (RA), revealed varying concentrations of RA in the different MEL extracts. RA concentrations remained stable for at least 9 months and in feed three months after incorporating MEL extract. When incubated with chicken cell lines MEL extract showed potential metabolic activation and ability to stimulate immune functions but induced cytotoxicity at high concentrations. The original ex vivo model of inflammation developed on chicken blood cells enabled inflammation and oxidative stress biomarkers to be expressed and revealed antioxidative and anti-inflammatory properties of blood cells from chickens fed MEL extract. The experimental model of chicken suboptimal starting conditions validated beneficial effects of MEL extract on the redox balance and also evidenced improved performance during the growth phase, a tendency for ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/34746291; PUBMED: 34746291; WOS: 000716533200001 |
| DOI: |
10.3389/fvets.2021.759456 |
| Availability: |
https://hal.inrae.fr/hal-03468715; https://hal.inrae.fr/hal-03468715v1/document; https://hal.inrae.fr/hal-03468715v1/file/2021_Travel_Front-Vet-Sci_vol-8_art-759456.pdf; https://doi.org/10.3389/fvets.2021.759456 |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.BE0A861E |
| Database: |
BASE |