| Title: |
Bisphenol S impairs oestradiol secretion during In vitro basal folliculogenesis in a mono-ovulatory species model |
| Authors: |
Vignault, Claire; Cadoret, Véronique; Jarrier-Gaillard, Peggy; Papillier, Pascal; Téteau, Ophélie; Desmarchais, Alice; Uzbekova, Svetlana; Binet, Aurélien; Guerif, Fabrice; Elis, Sébastien; Maillard, Virginie |
| Contributors: |
Physiologie de la reproduction et des comportements Nouzilly (PRC); Institut Français du Cheval et de l'Equitation Saumur (IFCE)-Université de Tours (UT); NEOLAiA European University = Université Européenne NEOLAÏA (NEOLAiA)-NEOLAiA European University = Université Européenne NEOLAÏA (NEOLAiA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Centre Hospitalier Régional Universitaire de Tours (CHRU Bretonneau); Region Centre-Val de Loire (APR IA 2019-00134937 PERFIDE); ANR-18-CE34-0011,MAMBO,Impact du métabolisme sur les effets ovariens du bisphenol S(2018) |
| Source: |
ISSN: 2305-6304 ; Toxics ; https://hal.inrae.fr/hal-03751976 ; Toxics, 2022, 10, pp.1-21. ⟨10.3390/toxics10080437⟩. |
| Publisher Information: |
CCSD; MDPI |
| Publication Year: |
2022 |
| Collection: |
Université François-Rabelais de Tours: HAL |
| Subject Terms: |
ewe; plasticiser; bisphenols; gene expression; hormonal secretions; follicular growth; endocrine disruptors; ovary; [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology; [SDV.SA]Life Sciences [q-bio]/Agricultural sciences; [SDE]Environmental Sciences; [SDV]Life Sciences [q-bio]; [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism; [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain |
| Description: |
International audience ; Bisphenol S (BPS) affects terminal folliculogenesis by impairing steroidogenesis in granulosa cells from different species. Nevertheless, limited data are available on its effects during basal folliculogenesis. In this study, we evaluate in vitro the effects of a long-term BPS exposure on a model of basal follicular development in a mono-ovulatory species. We cultured ovine preantral follicles (180–240 µm, n = 168) with BPS (0.1 µM (possible human exposure dose) or 10 µM (highdose)) and monitored antrum appearance and follicular survival and growth for 15 days. We measured hormonal secretions (oestradiol (at day 13 [D13]), progesterone and anti-Müllerian hormone [D15]) and expression of key follicular development and redox status genes (D15) in medium and whole follicles, respectively. BPS (0.1 µM) decreased oestradiol secretion compared with the control (-48.8%, p < 0.001), without significantly impairing antrum appearance, follicular survival and growth, anti-Müllerian hormone and progesterone secretion and target gene expression. Thus, BPS could also impair oestradiol secretion during basal folliculogenesis as it is the case during terminal folliculogenesis. It questions the use of BPS as a safe BPA substitute in the human environment. More studies are required to elucidate mechanisms of action of BPS and its effects throughout basal follicular development. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
WOS: 000845110100001 |
| DOI: |
10.3390/toxics10080437 |
| Availability: |
https://hal.inrae.fr/hal-03751976; https://hal.inrae.fr/hal-03751976v1/document; https://hal.inrae.fr/hal-03751976v1/file/Vignault%20et%20al.%202022%20Toxics%20BPS%20follic.pdf; https://doi.org/10.3390/toxics10080437 |
| Rights: |
https://about.hal.science/hal-authorisation-v1/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.BE7F954C |
| Database: |
BASE |