| Title: |
Identification of Two Eosinophil Subsets in Induced Sputum from Patients with Allergic Asthma According to CD15 and CD66b Expression |
| Authors: |
Elena Curto; Éder F. Mateus-Medina; Astrid Crespo-Lessmann; Rubén Osuna-Gómez; Cristina Ujaldón-Miró; Alba García-Moral; Paula Galván-Blasco; Lorena Soto-Retes; David Ramos-Barbón; Vicente Plaza |
| Source: |
International Journal of Environmental Research and Public Health, Vol 19, Iss 13400, p 13400 (2022) |
| Publisher Information: |
MDPI AG |
| Publication Year: |
2022 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
allergic asthma; eosinophil subsets; flow cytometry; induced sputum; Medicine |
| Description: |
Two subsets of eosinophils have been described: resident eosinophils with homeostatic functions (rEOS) in healthy subjects and in patients with nonallergic eosinophilic asthma, and inflammatory eosinophils (iEOS) in blood and lung samples from patients with allergic asthma. We explored if it would be possible to identify different subsets of eosinophils using flow cytometry and the gating strategy applied to induced sputum. We conducted an observational cross-sectional single-center study of 62 patients with persistent allergic asthma. Inflammatory cells from induced sputum samples were counted by light microscopy and flow cytometry, and cytokine levels in the supernatant were determined. Two subsets of eosinophils were defined that we call E1 (CD66b-high and CD15-high) and E2 (CD66b-low and CD15-low). Of the 62 patients, 24 were eosinophilic, 18 mixed, 10 paucigranulocytic, and 10 neutrophilic. E1 predominated over E2 in the eosinophilic and mixed patients (20.86% vs. 6.27% and 14.42% vs. 4.31%, respectively), while E1 and E2 were similar for neutrophilic and paucigranulocytic patients. E1 correlated with IL-5, fractional exhaled nitric oxide, and blood eosinophils. While eosinophil subsets have been identified for asthma in blood, we have shown that they can also be identified in induced sputum. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://www.mdpi.com/1660-4601/19/20/13400; https://doaj.org/toc/1661-7827; https://doaj.org/toc/1660-4601; https://doaj.org/article/52c0dc51a9ff4ce087241e5ff2df4e26 |
| DOI: |
10.3390/ijerph192013400 |
| Availability: |
https://doi.org/10.3390/ijerph192013400; https://doaj.org/article/52c0dc51a9ff4ce087241e5ff2df4e26 |
| Accession Number: |
edsbas.BEAB7C64 |
| Database: |
BASE |