| Title: |
96 sample parallel acoustic fragmentation for high throughput next generation sequencing library preparation |
| Authors: |
Mehrab-Mohseni, Marjan; Bautista, Kathlyne Jayne B.; Liu, Yusha; Maldonado-Rosado, Erika E.; Zheng, Haofan; Niederhuber, Matthew J.; Rosen, Jonathan D.; Carroll, Michell D.; Velasco, Brian; Tsuruta, James K.; Kim, Jinwook; Marron, James S.; Haaland, Perry D.; Kasoji, Sandeep K.; Mieczkowski, Piotr A.; Hepperla, Austin J.; Dayton, Paul A.; Pattenden, Samantha G. |
| Source: |
PLOS One, 21(2) |
| Publisher Information: |
PLOS |
| Publication Year: |
2026 |
| Collection: |
Carolina Digital Repository (UNC - University of North Carolina) |
| Subject Terms: |
Blast crisis; locomotion; Important; Necroptosis; Clinical trial; fMRI; PDGFRα; Cervical; Cancer; Targeted therapies; Breast cancer; Superconducting properties and materials; STING agonists; Antibacterial activity; head size; Women; Large language model; Beta-amyloid; Schiff base; Vaccines; cell tracking; Microbiome; Ginkgo biloba L. leaves extract; gemcitabine; Reactive arthritis; Nursing; spinal cord injury; Rhizobial symbiosis; Cancer genetics; Health Education |
| Description: |
Random, unbiased fragmentation of genomic DNA is necessary for next generation sequencing (NGS), yet the process of DNA fragmentation is still a bottleneck in NGS pipelines. A technology that increases the efficiency and consistency of this step will be highly desirable for both research laboratories and in clinical diagnostics. Previously, we reported the development of a novel cavitation enhancement reagent that dramatically decreases the time and acoustic energy required for genomic DNA fragmentation. The inclusion of this reagent in standard protocols facilitates highly efficient sonication enabling the use of widely available and inexpensive equipment, including water bath-based sonicators. Here, we report that cavitation enhancement facilitates parallel fragmentation of up to 96 samples of genomic DNA in a modified sonication device. The parallel processing of multiple samples significantly reduces processing time, while maintaining fragment range reproducibility and preserving DNA quality for NGS. Consequently, this system removes a key bottleneck in standard NGS pipelines and facilitates efforts toward research and personalized genomics. |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| Relation: |
https://cdr.lib.unc.edu/downloads/tq57p7026?file=thumbnail; https://cdr.lib.unc.edu/downloads/tq57p7026 |
| DOI: |
10.17615/8787-d398 |
| Availability: |
https://doi.org/10.17615/8787-d398; https://cdr.lib.unc.edu/downloads/tq57p7026?file=thumbnail; https://cdr.lib.unc.edu/downloads/tq57p7026 |
| Rights: |
http://rightsstatements.org/vocab/InC/1.0/ ; http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.BEC5D6EC |
| Database: |
BASE |