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Speckled-egg staining appearance of cryptococcal osteomyelitis in an immunocompetent patient

Title: Speckled-egg staining appearance of cryptococcal osteomyelitis in an immunocompetent patient
Authors: Stack, Matthew Alexander; Lavik, John-Paul; Schneider, Jack G.; Cross, Brynne E.
Contributors: Pathology and Laboratory Medicine, School of Medicine
Source: PMC
Publisher Information: Elsevier
Publication Year: 2025
Collection: Indiana University - Purdue University Indianapolis: IUPUI Scholar Works
Subject Terms: Cryptococcus neoformans; Histochemical staining; Cryptococcal osteomyelitis; Immunocompetent
Description: Introduction: Cryptococcal infections are typically thought of as occurring in immunocompromised patients, such as patients with HIV/AIDS, solid organ transplant recipients, or patients with rheumatologic diseases that require immunomodulatory therapy. Moreover, Cryptococcus spp. classically appear as variably-sized yeasts with narrow-based budding surrounded by a thick polysaccharide capsule. However, cryptococcal infections are being increasingly reported in atypical hosts, at times with non-characteristic histochemical staining appearances. Herein, we report a case of cryptococcal osteomyelitis in an otherwise immunocompetent individual that had a "speckled-egg" staining appearance on direct-smear Gram stain. Case: The patient is an otherwise healthy 89-year-old male with a past medical history notable only for hypertension who presented with progressive left-sided neck pain that became worse despite lidocaine trigger point injections; imaging was obtained and revealed a C1-C2 prevertebral abscess, C2-C4 osteomyelitis, and a small C2-C4 abscess. An aspiration biopsy from one of the cervical abscesses grew Cryptococcus neoformans. Despite prompt initiation of liposomal amphotericin B as soon as the organism was suspected, the patient's mentation declined with associated progression of weakness in his upper and lower extremities. The patient was ultimately transitioned to comfort care. Conclusions: Unconventional presentations of cryptococcal disease are becoming increasingly recognized in seemingly immunocompetent patients. Our case was unique given that it occurred in a patient who appeared to be immunocompetent and the Gram stain showed a speckled-egg staining pattern that alone was not distinctive for cryptococcal yeasts. Despite the patient's lack of any classic comorbidities associated with invasive cryptococcal disease, his advanced age was likely a risk factor.
Document Type: article in journal/newspaper
File Description: application/pdf
Language: English
Relation: IDCases; https://hdl.handle.net/1805/46557
Availability: https://hdl.handle.net/1805/46557
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International ; https://creativecommons.org/licenses/by-nc-nd/4.0/
Accession Number: edsbas.BF87B498
Database: BASE