| Title: |
Vitamin C and E supplementation effects on secretory and molecular aspects of vascular endothelial growth factor derived from peritoneal fluids of patients with endometriosis |
| Authors: |
Hossein Ansariniya; Hossein Hadinedoushan; Atiyeh Javaheri; Fateme Zare |
| Source: |
Journal of Obstetrics and Gynaecology, Vol 39, Iss 8, Pp 1137-1142 (2019) |
| Publisher Information: |
Taylor & Francis Group |
| Publication Year: |
2019 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
endometriosis; gene expression; vascular endothelial growth factor; vitamin c; vitamin e; Gynecology and obstetrics; RG1-991 |
| Description: |
Endometriosis is an extremely heterogeneous disease and affects about ten percent of the female population during their reproductive years. Recent studies showed that endometriosis is an angiogenesis-dependent disease. Peritoneal macrophages are a well-characterised source of vascular endothelial growth factor (VEGF). The aim of this study was to determine the VEGF gene expression and production in peritoneal macrophages of patients with endometriosis under the effects of vitamins C and E in comparison with control. The lab trial study carried out on 50 patients undergoing laparoscopy and peritoneal fluid samples were collected from them. We compared the VEGF gene expression and production in peritoneal macrophages among groups by using real-time polymerase chain reaction and enzyme-linked immunosorbent assay methods, respectively. Our results showed that gene expressions influenced by vitamin C increased in different concentrations and incubation times, except for the incubation time after 48 h. In the case of vitamin E, this was evident with the exception of vitamin E 50 μM after 24 h and vitamin E 100 μM after 48 h. Our findings indicated that vitamin C and E in different concentrations and incubation times altered VEGF gene expression in the peritoneal macrophages but they had not affected on VEGF productions.Impact statement What is already known on this subject? Previous studies showed that antioxidants play a key role in the inhibition of oxidative stress-induced damages and the reduction of pelvic pain in patients with endometriosis. Vitamin E and vitamin C are the main components in neutralising free radicals. Also, antioxidant consumption such as vitamin C and vitamin E in women with endometriosis showed an inverse correlation between antioxidant intake and endometriosis pathology. What do the results of this study add? Vitamin C and E in different concentrations and times of incubation altered vascular endothelial growth factor gene expression and production in peritoneal macrophages. What are the ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
http://dx.doi.org/10.1080/01443615.2019.1601167; https://doaj.org/toc/0144-3615; https://doaj.org/toc/1364-6893; https://doaj.org/article/39c266cf2c064b4d93730982cdb0612d |
| DOI: |
10.1080/01443615.2019.1601167 |
| Availability: |
https://doi.org/10.1080/01443615.2019.1601167; https://doaj.org/article/39c266cf2c064b4d93730982cdb0612d |
| Accession Number: |
edsbas.BFD0E47D |
| Database: |
BASE |