| Title: |
Double-negative B cells and DNASE1L3 colocalise with microbiota in gut-associated lymphoid tissue. |
| Authors: |
Montorsi, L; Pitcher, MJ; Zhao, Y; Dionisi, C; Demonti, A; Tull, TJ; Dhami, P; Ellis, RJ; Bishop, C; Sanderson, JD; Jain, S; D'Cruz, D; Gibbons, DL; Winkler, TH; Bemark, M; Ciccarelli, FD; Spencer, J |
| Publisher Information: |
Springer Nature |
| Publication Year: |
2024 |
| Collection: |
Queen Mary University of London: Queen Mary Research Online (QMRO) |
| Subject Terms: |
Animals; Female; Humans; Male; Mice; B-Lymphocytes; Dendritic Cells; Endodeoxyribonucleases; Gastrointestinal Microbiome; Intestinal Mucosa; Lymphoid Tissue; Inbred C57BL |
| Description: |
Intestinal homeostasis is maintained by the response of gut-associated lymphoid tissue to bacteria transported across the follicle associated epithelium into the subepithelial dome. The initial response to antigens and how bacteria are handled is incompletely understood. By iterative application of spatial transcriptomics and multiplexed single-cell technologies, we identify that the double negative 2 subset of B cells, previously associated with autoimmune diseases, is present in the subepithelial dome in health. We show that in this location double negative 2 B cells interact with dendritic cells co-expressing the lupus autoantigens DNASE1L3 and C1q and microbicides. We observe that in humans, but not in mice, dendritic cells expressing DNASE1L3 are associated with sampled bacteria but not DNA derived from apoptotic cells. We propose that fundamental features of autoimmune diseases are microbiota-associated, interacting components of normal intestinal immunity. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
Nature Communications; https://qmro.qmul.ac.uk/xmlui/handle/123456789/100950 |
| DOI: |
10.1038/s41467-024-48267-4 |
| Availability: |
https://qmro.qmul.ac.uk/xmlui/handle/123456789/100950; https://doi.org/10.1038/s41467-024-48267-4 |
| Rights: |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/ licenses/by/4.0/. ; © The Author(s) 2024 |
| Accession Number: |
edsbas.BFDA6BE |
| Database: |
BASE |