| Title: |
Event-based vision sensor for fast and dense single-molecule localization microscopy |
| Authors: |
Cabriel, Clément; Monfort, Tual; Specht, Christian; Izeddin, Ignacio |
| Contributors: |
Institut Langevin - Ondes et Images (UMR7587) (IL); Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris); Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS); Institut de la Vision; Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS); Centre d'investigation clinique Quinze-Vingts CHNO (CIC1423 - CIC QUINZE-VINGTS); Institut Hospitalo-Universitaire FOReSIGHT (IHU FOReSIGHT); Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts (CHNO); Sorbonne Université (SU)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts (CHNO); Sorbonne Université (SU)-Sorbonne Université (SU); Maladies et hormones du système nerveux (DHNS); Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay; ANR-20-CE45-0023,ABC4M,Microscopie multimodale assistée par calcul Bayésien approximé pour explorer la mobilité nucléaire des facteurs de transcription(2020); ANR-20-CE24-0021,SP-Tunnel-OHG,Génération d'harmoniques d'ordre élevé dans les jonctions tunnel plasmoniques(2020) |
| Source: |
ISSN: 1749-4885. |
| Publisher Information: |
CCSD; Nature Publishing Group |
| Publication Year: |
2026 |
| Subject Terms: |
[SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic; [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry; Molecular Biology/Biophysics; [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging |
| Description: |
International audience ; Single-molecule localization microscopy (SMLM) enables revealing crucial insights into cellular structures and processes at the single molecule level. However, SMLM is often hampered by limited temporal resolution and the fixed frame rate of the acquisition. Here, we present a new approach to SMLM data acquisition and processing based on an affordable event-based sensor. This type of sensor reacts to changes of light intensity rather than integrating photons during each frame exposure time. Each pixel works independently and returns a signal only when an intensity change is detected. In comparison to video acquisition using traditional EMCCD or sCMOS cameras, the event-based sensor provides higher temporal resolution and throughput on the position of blinking molecules. We demonstrate event-based SMLM super-resolution imaging on biological samples with spatial resolution on par with EMCCD or sCMOS performance, while registering only the on and off-switching of blinking molecules. We use event-based SMLM to perform very dense single molecule imaging, where frame-based cameras experience major limitations. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1038/s41566-023-01308-8 |
| Availability: |
https://hal.science/hal-05568913; https://hal.science/hal-05568913v1/document; https://hal.science/hal-05568913v1/file/Evb_manuscript_review_review_review_shortened-1.pdf; https://hal.science/hal-05568913v1/file/F1.pdf; https://hal.science/hal-05568913v1/file/F2.pdf; https://hal.science/hal-05568913v1/file/F3.pdf; https://hal.science/hal-05568913v1/file/F4.pdf; https://doi.org/10.1038/s41566-023-01308-8 |
| Rights: |
https://creativecommons.org/licenses/by-nc/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.BFEB5D7E |
| Database: |
BASE |