| Title: |
Characterization of broad host range bacteriophages vKpIN31 and vKpIN32 against hospital-acquired Klebsiella pneumoniae in Dakar, Senegal |
| Authors: |
Ndiaye, Issa; Debarbieux, Laurent; Sow, Ousmane; Ba, Bissoume Sambe; Diagne, Moussa Moise; Cissé, Abdoulaye; Fall, Cheikh; Dièye, Baidy; Dieng, Assane; Diop, Amadou; Dieye, Yakhya; Dia, Ndongo; Constantin de Magny, Guillaume; Seck, Abdoulaye |
| Contributors: |
Institut Pasteur de Dakar; Pasteur Network (Réseau International des Instituts Pasteur); Bactériophage, bactérie, hôte - Bacteriophage, bacterium, host; Université Paris Cité (UPCité)-Microbiologie Intégrative et Moléculaire (UMR6047); Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS); Organisation Mondiale de la Santé / World Health Organization Office Genève, Suisse (OMS / WHO); Hôpital des Enfants Albert Royer Dakar, Sénégal (HEAR); Centre Hospitalier National et Universitaire de Fann-Dakar Dakar, Sénégal; Hôpital Aristide-Le-Dantec; Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC); Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD Occitanie )-Université de Montpellier (UM); Montpellier Ecology and Evolution of Disease Network Montpellier, France (MEEDiN); Université Cheikh Anta Diop de Dakar Sénégal (UCAD) |
| Source: |
ISSN: 2045-2322. |
| Publisher Information: |
CCSD; Nature Publishing Group |
| Publication Year: |
2026 |
| Subject Terms: |
Klebsiella pneumoniae; HAIs; phage therapy; vKpIN32; vKpIN31; bacteriophages; MDR; [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology; [SDV.EE.IEO]Life Sciences [q-bio]/Ecology; environment/Symbiosis; [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases; [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology |
| Description: |
International audience ; Klebsiella pneumoniae, a common gut colonizer, has become a major opportunistic pathogen, especially with the rise of multidrug-resistant (MDR) strains. This study aimed to characterize two lytic bacteriophages against MDR K. pneumoniae strains isolated from hospital associated infections in Senegal. Among 28 MDR K. pneumoniae strains tested, phage vKpIN31 effectively lyse 15 strains encompassing 12 distinct K locus types. While phage vKpIN32 lysed 12 strains with 9 different K locus types, demonstrating broad host range activity. The isolated phages exhibited thermal and pH stability. One-step growth analysis revealed a latent period of 25 and 20 minutes and burst sizes of 281 and 246 PFU/cell for vKpIN31 and vKpIN32 respectively. The in vitro lytic activity of phages vKpIN31 and vKpIN32 at different multiplicity of infection (1, 10⁻¹, and 10⁻³) revealed variable lysis efficacy against three K. pneumoniae strains (KP6, KP7, and KP17), with the highest effectiveness observed at an MOI of 10⁻³ for both phages. Also, combination of both phages as cocktail led to improved efficacy against the targeted strains Also, both phages significantly reduced biofilm levels, from 18.6% to 67.9% for 24-hour mature biofilms and from 18.1% to 58.7% for 48-hour mature biofilms. Genomic analysis identified both phages as linear dsDNA viruses belonging to the Caudoviricetes class, and Sugarlandvirus sugarland species. No genes associated with a temperate life cycle, integrases, transposable elements, antibiotic resistance, or bacterial virulence were detected in their genomes. These findings highlight vKpIN31 and vKpIN32 as promising candidates for phage therapy. Additionally, their potential extends to serving as sources for antibacterial and antibiofilm agents, signifying their clinical relevance and therapeutic potential. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/41390877; PUBMED: 41390877 |
| DOI: |
10.1038/s41598-025-32351-w |
| Availability: |
https://hal.science/hal-05446682; https://hal.science/hal-05446682v2/document; https://hal.science/hal-05446682v2/file/Article%20Issa%20Scientific%20Reports.pdf; https://doi.org/10.1038/s41598-025-32351-w |
| Rights: |
https://creativecommons.org/licenses/by-nc-nd/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.C0EC70B3 |
| Database: |
BASE |