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Jianzheng Wang,1,* Qingli Li,1,* Xiaojiao Cheng,1 Baiwen Zhang,1 Jiacheng Lin,1 Yao Tang,1 Fuli Li,1 Chung S Yang,2 Timothy C Wang,3 Shuiping Tu1 1Department of Oncology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200127, People’s Republic of China; 2Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA; 3Department of Medicine, College of Physicians & Surgeons, Columbia University, New York, NY 10032, USA*These authors contributed equally to this workCorrespondence: Shuiping Tu; Jianzheng WangDepartment of Oncology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, People’s Republic of ChinaTel +862168385623; +16696079698Email tushuiping@yahoo.com; 46962384@qq.comBackground: Murine bone marrow-derived myofibroblasts (BMFs) have previously been shown to promote gastric cancer growth. However, whether BMFs promote gastric cancer cell metastasis remains largely unknown.Methods: Wound healing assay, Transwell invasion and migration assay and 3D organotypic co-culture systems were conducted to study the effects of BMFs on invasion and migration of gastric cancer cells and the invasion and migration ability of gastric cancer stem cell-like cells (CSC-LCs) induced by BMFs. We employed two animal model to study the role of BMFs on the in vivo metastasis of gastric cancer cells and the metastatic ability of gastric BMF-induced CSC-LCs. A human gastric cancer tissue microarray and TCGA gastric cancer database were analysed to study the relationship between the expression of IL-6 and TGF-β 1 and clinicopathological characteristics and survival in gastric cancer.Results: We found that BMFs promoted the in vitro migration and invasion of gastric cancer cells. BMFs promoted liver, lung, subcutaneous, and splenic metastases of MKN28 cells in the spleen injection liver metastasis model and co-injection of caudal vein (IOCV) mouse model. BMFs reprogrammed non-gastric cancer stem ... |