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High-Confidence Placement of Fragments into Electron Density Using Anomalous Diffraction—A Case Study Using Hits Targeting SARS-CoV-2 Non-Structural Protein 1

Title: High-Confidence Placement of Fragments into Electron Density Using Anomalous Diffraction—A Case Study Using Hits Targeting SARS-CoV-2 Non-Structural Protein 1
Authors: Ma, Shumeng; Mykhaylyk, Vitaliy; Bowler, Matthew W; Pinotsis, Nikos; Kozielski, Frank
Source: International Journal of Molecular Sciences , 24 (13) , Article 11197. (2023)
Publisher Information: MDPI AG
Publication Year: 2023
Collection: University College London: UCL Discovery
Subject Terms: SARS-CoV-2; COVID-19; non-structural proteins; nsp1; tunable wavelength; fragment orientation; anomalous difference Fourier map
Description: The identification of multiple simultaneous orientations of small molecule inhibitors binding to a protein target is a common challenge. It has recently been reported that the conformational heterogeneity of ligands is widely underreported in the Protein Data Bank, which is likely to impede optimal exploitation to improve affinity of these ligands. Significantly less is even known about multiple binding orientations for fragments (
Document Type: article in journal/newspaper
File Description: text
Language: English
Relation: https://discovery.ucl.ac.uk/id/eprint/10173847/
Availability: https://discovery.ucl.ac.uk/id/eprint/10173847/1/ijms-24-11197.pdf; https://discovery.ucl.ac.uk/id/eprint/10173847/
Rights: open
Accession Number: edsbas.C10F16EF
Database: BASE